ArticleProceedings of the National Academy of Sciences of the United States of America2013
Diacylglycerol promotes centrosome polarization in T cells via reciprocal localization of dynein and myosin II.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 100 citations in OpenAlex.
- Mechanoregulation of lymphocyte cytotoxicity.Nature reviews. Immunology · 2025Review
- CD99-mediated immunological synapse formation potentiates CAR-T cell function.Nature communications · 2025Article
- Dynein-driven regulation of postsynaptic membrane architecture and synaptic function.Journal of cell science · 2025Article
- Suppression of non-muscle myosin II boosts T cell cytotoxicity against tumors.Science advances · 2024Article
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8Frontiers in immunology · 2024Article
- Apoptotic contraction drives target cell release by cytotoxic T cells.Nature immunology · 2023Article
- Extracellular vesicles and microvilli in the immune synapse.Frontiers in immunology · 2023Review
- Stochastic model of T cell repolarization during target elimination (II).Biophysical journal · 2022Article
- Cell polarity regulators, multifunctional organizers of lymphocyte activation and function.Biomedical journal · 2022Review
- T Lymphocyte and CAR-T Cell-Derived Extracellular Vesicles and Their Applications in Cancer Therapy.Cells · 2022Review
- Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Force Generation.Frontiers in immunology · 2022Article
- Actomyosin dynamics modulate microtubule deformation and growth during T-cell activation.Molecular biology of the cell · 2021Article
- LFA-1 and kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors.Journal of cell science · 2021Article
- Dynamic Microtubule Arrays in Leukocytes and Their Role in Cell Migration and Immune Synapse Formation.Frontiers in cell and developmental biology · 2021Review
- Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse.Frontiers in immunology · 2021Article
- Role of Actin Cytoskeleton Reorganization in Polarized Secretory Traffic at the Immunological Synapse.Frontiers in cell and developmental biology · 2021Review
- Kinesin-4 KIF21B limits microtubule growth to allow rapid centrosome polarization in T cells.eLife · 2020Article
- Interplay Between SNX27 and DAG Metabolism in the Control of Trafficking and Signaling at the IS.International journal of molecular sciences · 2020Review
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Centrosome reorientation to the immunological synapse maintains the specificity of T-cell effector function by facilitating the directional release of cytokines and cytolytic factors toward the antigen-presenting cell. This polarization response is driven by the localized accumulation of diacylglycerol, which recruits multiple protein kinase (PK)C isozymes to the synaptic membrane. Here, we used T-cell receptor (TCR) photoactivation and imaging methodology to demonstrate that PKCs control centrosome dynamics through the reciprocal localization of two motor complexes, dynein and nonmuscle myosin (NM)II. Dynein accumulated in the region of TCR stimulation, whereas NMII clustered in the back of the cell, behind the polarizing centrosome. PKC activity, which shaped both dynein and NMII accumulation within this framework, controlled NMII localization directly by phosphorylating inhibitory sites within the myosin regulatory light chain, thereby suppressing NMII clustering in the region of TCR stimulation. Concurrently, phosphorylation of distinct sites within myosin regulatory light chain by Rho kinase drove NMII clustering in areas behind the centrosome. These results reveal a role for NMII in T-cell polarity and demonstrate how it is regulated by upstream signals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.