ArticleProceedings of the National Academy of Sciences of the United States of America2013
Mn(II,III) oxidation and MnO2 mineralization by an expressed bacterial multicopper oxidase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Comparative transcriptomics and scarless genome editing uncover a periplasmicApplied and environmental microbiology · 2026Article
- Multi-omic landscape of Mn(Ⅱ) oxidation in Achromobacter pulmonis ss21: from multicopper oxidase to metabolic support electron transfer.World journal of microbiology & biotechnology · 2026Article
- Synergy between reactive manganese species and enzymes drives litter decomposition hotspots.Nature communications · 2026Article
- DistinguishingmSystems · 2026Article
- Deciphering Cu(II) EPR in biological buffers: Implications for copper protein studies.Journal of inorganic biochemistry · 2026Article
- Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization.NPJ biofilms and microbiomes · 2025Article
- Biogenic Mn Oxide Generation and Mn(II) Removal by a Manganese Oxidizing Bacterium Bacillus sp. Strain M2.Applied biochemistry and biotechnology · 2024Article
- Redox-Driven Formation of Mn(III) in Ice.Environmental science & technology · 2024Article
- Advances in Research on Bacterial Oxidation of Mn(II): A Visualized Bibliometric Analysis Based on CiteSpace.Microorganisms · 2024Review
- Mineral-mediated stability of organic carbon in soil and relevant interaction mechanisms.Eco-Environment & Health · 2024Review
- Laboratory Evolution of Metalloid Reductase Substrate Recognition and Nanoparticle Product Size.ACS chemical biology · 2024Article
- Picolinic Acid-Mediated Catalysis of Mn(II) for Peracetic Acid Oxidation Processes: Formation of High-Valent Mn Species.Environmental science & technology · 2023Article
- Distinguishing homogeneous advanced oxidation processes in bulk water from heterogeneous surface reactions in organic oxidation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Biomineralized Manganese Oxide Nanoparticles Synergistically Relieve Tumor Hypoxia and Activate Immune Response with Radiotherapy in Non-Small Cell Lung Cancer.Nanomaterials (Basel, Switzerland) · 2022Article
- Mineralogical and Genomic Constraints on the Origin of Microbial Mn Oxide Formation in Complexed Microbial Community at the Terrestrial Hot Spring.Life (Basel, Switzerland) · 2022Article
- A soil-borne Mn(II)-oxidizing bacterium of Providencia sp. exploits a strategy of superoxide production coupled to hydrogen peroxide consumption to generate Mn oxides.Archives of microbiology · 2022Article
- Article
- Forced Biomineralization: A Review.Biomimetics (Basel, Switzerland) · 2021Review
- Synthesis of MnO/C/CoScience and technology of advanced materials · 2021Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Reactive Mn(IV) oxide minerals are ubiquitous in the environment and control the bioavailability and distribution of many toxic and essential elements and organic compounds. Their formation is thought to be dependent on microbial enzymes, because spontaneous Mn(II) to Mn(IV) oxidation is slow. Several species of marine Bacillus spores oxidize Mn(II) on their exosporium, the outermost layer of the spore, encrusting them with Mn(IV) oxides. Molecular studies have identified the mnx (Mn oxidation) genes, including mnxG, encoding a putative multicopper oxidase (MCO), as responsible for this two-electron oxidation, a surprising finding because MCOs only catalyze single-electron transfer reactions. Characterization of the enzymatic mechanism has been hindered by the lack of purified protein. By purifying active protein from the mnxDEFG expression construct, we found that the resulting enzyme is a blue (absorption maximum 590 nm) complex containing MnxE, MnxF, and MnxG proteins. Further, by analyzing the Mn(II)- and (III)-oxidizing activity in the presence of a Mn(III) chelator, pyrophosphate, we found that the complex facilitates both electron transfers from Mn(II) to Mn(III) and from Mn(III) to Mn(IV). X-ray absorption spectroscopy of the Mn mineral product confirmed its similarity to Mn(IV) oxides generated by whole spores. Our results demonstrate that Mn oxidation from soluble Mn(II) to Mn(IV) oxides is a two-step reaction catalyzed by an MCO-containing complex. With the purification of active Mn oxidase, we will be able to uncover its mechanism, broadening our understanding of Mn mineral formation and the bioinorganic capabilities of MCOs.
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