ArticleThe Journal of biological chemistry2013
Binding and movement of individual Cel7A cellobiohydrolases on crystalline cellulose surfaces revealed by single-molecule fluorescence imaging.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The carbohydrate-binding module of TrCel7A aids in navigating hemicellulose barriers in plant cell walls.The Journal of biological chemistry · 2026Article
- Slowdown of Enzymatic Cellulose Conversion Emerges from Cellulase Mode of Action.ACS catalysis · 2026Article
- Investigation Into the Role of Reductants and Cosubstrates in Lytic Polysaccharide Monooxygenase Thermothielavioides terrestris AA9E Binding to Cellulose by Single-Molecule Imaging.Biotechnology and bioengineering · 2026Article
- Are cellulases slow? Kinetic and thermodynamic limitations for enzymatic breakdown of cellulose.BBA advances · 2025Review
- Thermobifida fusca Cel6B moves bidirectionally while processively degrading cellulose.Biotechnology for biofuels and bioproducts · 2024Article
- Impact of Synergy Partner Cel7B on Cel7A Binding Rates: Insights from Single-Molecule Data.The journal of physical chemistry. B · 2024Article
- Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A fromThe Journal of biological chemistry · 2020Article
- Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues.The Journal of biological chemistry · 2020Article
- Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose.Biotechnology for biofuels · 2020Article
- Distinct Substrate Specificities and Electron-Donating Systems of Fungal Lytic Polysaccharide Monooxygenases.Frontiers in microbiology · 2018Review
- Visualising recalcitrance by colocalisation of cellulase, lignin and cellulose in pretreated pine biomass using fluorescence microscopy.Scientific reports · 2017Article
- Stimulation and inhibition of enzymatic hydrolysis by organosolv lignins as determined by zeta potential and hydrophobicity.Biotechnology for biofuels · 2017Article
- Inter-domain Synergism Is Required for Efficient Feeding of Cellulose Chain into Active Site of Cellobiohydrolase Cel7A.The Journal of biological chemistry · 2016Article
- Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose.The Journal of biological chemistry · 2016Article
- Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution.Biotechnology for biofuels · 2016Article
- Cellobiohydrolase 1 from Trichoderma reesei degrades cellulose in single cellobiose steps.Nature communications · 2015Article
- Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases.The Journal of biological chemistry · 2015Article
- The predominant molecular state of bound enzyme determines the strength and type of product inhibition in the hydrolysis of recalcitrant polysaccharides by processive enzymes.The Journal of biological chemistry · 2015Article
- Probing substrate interactions in the active tunnel of a catalytically deficient cellobiohydrolase (Cel7).The Journal of biological chemistry · 2015Article
- A comparative biochemical investigation of the impeding effect of C1-oxidizing LPMOs on cellobiohydrolases.The Journal of biological chemistryArticle
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Authors and funding
7 authors.
Funding
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Abstract
The efficient catalytic conversion of biomass to bioenergy would meet a large portion of energy requirements in the near future. A crucial step in this process is the enzyme-catalyzed hydrolysis of cellulose to glucose that is then converted into fuel such as ethanol by fermentation. Here we use single-molecule fluorescence imaging to directly monitor the movement of individual Cel7A cellobiohydrolases from Trichoderma reesei (TrCel7A) on the surface of insoluble cellulose fibrils to elucidate molecular level details of cellulase activity. The motion of multiple, individual TrCel7A cellobiohydrolases was simultaneously recorded with ∼15-nm spatial resolution. Time-resolved localization microscopy provides insights on the activity of TrCel7A on cellulose and informs on nonproductive binding and diffusion. We measured single-molecule residency time distributions of TrCel7A bound to cellulose both in the presence of and absence of cellobiose the major product and a potent inhibitor of Cel7A activity. Combining these results with a kinetic model of TrCel7A binding provides microscopic insight into interactions between TrCel7A and the cellulose substrate.
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