ArticleObesity (Silver Spring, Md.)2014
Lipid in skeletal muscle myotubes is associated to the donors' insulin sensitivity and physical activity phenotypes.
Article in Obesity (Silver Spring, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary, and Type 2 Diabetic Males.The Journal of clinical endocrinology and metabolism · 2015Trial
- Preclinical research in obesity-associated metabolic diseases using in vitro, multicellular, and non-mammalian models.Journal of physiology and biochemistry · 2025Review
- Sphingolipid and Trimethylamine-N-Oxide (TMAO) Levels in Women with Obesity after Combined Physical Training.Metabolites · 2024Article
- Experimental cell models of insulin resistance: overview and appraisal.Frontiers in endocrinology · 2024Review
- Acetate Does Not Affect Palmitate Oxidation and AMPK Phosphorylation in Human Primary Skeletal Muscle Cells.Frontiers in endocrinology · 2021Article
- Overfeeding and Substrate Availability, But Not Age or BMI, Alter Human Satellite Cell Function.Nutrients · 2020Article
- Sphingolipid Metabolism and Signaling in Skeletal Muscle: From Physiology to Physiopathology.Frontiers in endocrinology · 2020Review
- Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects.Scientific reports · 2018Article
- Skeletal muscle ceramides and daily fat oxidation in obesity and diabetes.Metabolism: clinical and experimental · 2018Article
- Intramyocellular Lipid Droplet Size Rather Than Total Lipid Content is Related to Insulin Sensitivity After 8 Weeks of Overfeeding.Obesity (Silver Spring, Md.) · 2017Article
- Intramyocellular ceramides and skeletal muscle mitochondrial respiration are partially regulated by Toll-like receptor 4 during hindlimb unloading.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Effect of serial cell passaging in the retention of fiber type and mitochondrial content in primary human myotubes.Obesity (Silver Spring, Md.) · 2015Article
- A Review of Mitochondrial-derived Fatty Acids in Epigenetic Regulation of Obesity and Type 2 Diabetes.Journal of nutritional health & food science · 2014Article
- Defect of insulin signal in peripheral tissues: Important role of ceramide.World journal of diabetes · 2014Review
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveThis study investigated the relationship between in vitro lipid content in myotubes and in vivo whole body phenotypes of the donors such as insulin sensitivity, intramyocellular lipids (IMCL), physical activity, and oxidative capacity. DESIGN AND
methodsSix physically active donors were compared to six sedentary lean and six T2DM. Lipid content was measured in tissues and myotubes by immunohistochemistry. Ceramides, triacylglycerols, and diacylglycerols (DAGs) were measured by LC-MS-MS and GC-FID. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (80 mU min⁻¹ m⁻²), maximal mitochondrial capacity (ATPmax) by ³¹P-MRS, physical fitness by VO₂max and physical activity level (PAL) by accelerometers.
resultsMyotubes cultured from physically active donors had higher lipid content (0.047 ± 0.003 vs. 0.032 ± 0.001 and 0.033 ± 0.001AU; P < 0.001) than myotubes from lean and T2DM donors. Lipid content in myotubes was not associated with IMCL in muscle tissue but importantly, correlated with in vivo measures of ATPmax (r = 0.74; P < 0.001), insulin sensitivity (r = 0.54; P < 0.05), type-I fibers (r = 0.50; P < 0.05), and PAL (r = 0.92; P < 0.0001). DAGs and ceramides in myotubes were inversely associated with insulin sensitivity (r = -0.55, r = -0.73; P < 0.05) and ATPmax (r = -0.74, r = -0.85; P < 0.01).
conclusionsThese results indicate that cultured human myotubes can be used in mechanistic studies to study the in vitro impact of interventions on phenotypes such as mitochondrial capacity, insulin sensitivity, and physical activity.
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Registered trials
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