Evidence map›Paper›PMID 23817127›Full record

ArticleNature methods2013

Improved tools for the Brainbow toolbox.

Dawen Cai, Kimberly B Cohen, Tuanlian Luo, Jeff W Lichtman, Joshua R Sanes

Abstract read
In one paragraph

Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed.

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  19. Lineage memory shapes viral resistance barriers in human skin.bioRxiv : the preprint server for biology · 2025
    Article
  20. Live-cell omics with Raman spectroscopy.Microscopy (Oxford, England) · 2025
    Review

49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dawen Cai1] Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
Kimberly B Cohen
Tuanlian Luo
Jeff W Lichtman
Joshua R Sanes

Funding

Next Generation Brainbow Transgenes for Neural Circuit AnalysisU24NS063931 · NINDS · HARVARD UNIVERSITY · PI SANES, JOSHUA R · 2009 to 2012
$2.3M
NINDS NIH HHS U24 NS063931
6 · The paper itself

Abstract

In the transgenic multicolor labeling strategy called 'Brainbow', Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors. This method has been adapted to non-neuronal cells in mice and to neurons in fish and flies, but its full potential has yet to be realized in the mouse brain. Here we present several lines of mice that overcome limitations of the initial lines, and we report an adaptation of the method for use in adeno-associated viral vectors. We also provide technical advice about how best to image Brainbow-expressing tissue.

Identifiers

PMID23817127
PMCPMC3713494

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.