ArticleNature methods2013
TALE-mediated modulation of transcriptional enhancers in vivo.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Chromatin enables precise and scalable gene regulation with factors of limited specificity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- SynNotch CAR-T cell, when synthetic biology and immunology meet again.Frontiers in immunology · 2025Review
- Identification of stress-induced epigenetic methylation onto dopamine D2 gene and neurological and behavioral consequences.Gene & protein in disease · 2024Article
- Competing constraints shape the nonequilibrium limits of cellular decision-making.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A synthetic synthesis to explore animal evolution and development.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Integration of Count Difference and Curve Similarity in Negative Regulatory Element Detection.Frontiers in genetics · 2022Article
- Epigenetics and addiction.Current opinion in neurobiology · 2019Review
- Programmable T7-based synthetic transcription factors.Nucleic acids research · 2018Article
- Rationally-engineered reproductive barriers using CRISPR & CRISPRa: an evaluation of the synthetic species concept in Drosophila melanogaster.Scientific reports · 2018Article
- The past and presence of gene targeting: from chemicals and DNA via proteins to RNA.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.Nature communications · 2017Article
- Article
- Towards quantitative analysis of gene regulation by enhancers.Epigenomics · 2017Review
- The effect of increasing numbers of repeats on TAL effector DNA binding specificity.Nucleic acids research · 2017Article
- From Reductionism to Holism: Toward a More Complete View of Development Through Genome Engineering.Advances in experimental medicine and biology · 2017Review
- Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system.Oncotarget · 2016Article
- Applying CRISPR-Cas9 tools to identify and characterize transcriptional enhancers.Nature reviews. Molecular cell biology · 2016Article
- FusX: A Rapid One-Step Transcription Activator-Like Effector Assembly System for Genome Science.Human gene therapy · 2016Article
- Quantitatively predictable control of Drosophila transcriptional enhancers in vivo with engineered transcription factors.Nature genetics · 2016Article
- Genome Editing in C. elegans and Other Nematode Species.International journal of molecular sciences · 2016Review
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Abstract
We tested whether transcription activator-like effectors (TALEs) could mediate repression and activation of endogenous enhancers in the Drosophila genome. TALE repressors (TALERs) targeting each of the five even-skipped (eve) stripe enhancers generated repression specifically of the focal stripes. TALE activators (TALEAs) targeting the eve promoter or enhancers caused increased expression primarily in cells normally activated by the promoter or targeted enhancer, respectively. This effect supports the view that repression acts in a dominant fashion on transcriptional activators and that the activity state of an enhancer influences TALE binding or the ability of the VP16 domain to enhance transcription. In these assays, the Hairy repression domain did not exhibit previously described long-range transcriptional repression activity. The phenotypic effects of TALER and TALEA expression in larvae and adults are consistent with the observed modulations of eve expression. TALEs thus provide a novel tool for detection and functional modulation of transcriptional enhancers in their native genomic context.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.