Evidence map›Paper›PMID 23816534›Full record

ArticleCellular signalling2013

Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.

Christina R Chow, Nobuchika Suzuki, Takeshi Kawamura, Takao Hamakubo, Tohru Kozasa

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular signalling, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. IPCellular and molecular life sciences : CMLS · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Christina R ChowDepartment of Pharmacology, University of Illinois at Chicago, 835 S. Wolcott Avenue Room E403 (m/c 868), Chicago, IL 60612, USA. crchow@uic.edu
Nobuchika Suzuki
Takeshi Kawamura
Takao Hamakubo
Tohru Kozasa
The University of Tokyo · JPUniversity of Illinois Chicago · US

Funding

Regulation of Rho family GTPases by heterotrimeric G proteinsR01GM061454 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI KOZASA, TOHRU, KREUTZ, BARRY S. · 1999 to 2014
$3.3M
Regulation of G Protein Signaling by RGS ProteinsR01GM074001 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI KOZASA, TOHRU · 2008 to 2011
$1.2M
NIGMS NIH HHS GM061454NIGMS NIH HHS GM074001NIGMS NIH HHS R01 GM061454NIGMS NIH HHS R01 GM074001
6 · The paper itself

Abstract

p115RhoGEF is a member of a family of Rho-specific guanine nucleotide exchange factors that also contains a regulator of G protein signaling homology domain (RH-RhoGEFs) that serves as a link between Gα13 signaling and RhoA activation. While the mechanism of regulation of p115RhoGEF by Gα13 is becoming well-known, the role of other regulatory mechanisms, such as post-translational modification or autoinhibition, in mediating p115RhoGEF activity is less well-characterized. Here, putative phosphorylation sites on p115RhoGEF are identified and characterized. Mutation of Ser(330) leads to a decrease in serum response element-mediated transcription as well as decreased activation by Gα13 in vitro. Additionally, this study provides the first report of the binding kinetics between full-length p115RhoGEF and RhoA in its various nucleotide states and examines the binding kinetics of phospho-mutant p115RhoGEF to RhoA. These data, together with other recent reports on regulatory mechanisms of p115RhoGEF, suggest that this putative phosphorylation site serves as a means for initiation or relief of autoinhibition of p115RhoGEF, providing further insight into the regulation of its activity.

Indexed as

Gene Expression RegulationProtein Processing, Post-TranslationalAmino Acid SequenceCell Line, TumorEscherichia coliGenes, ReporterGTP-Binding Protein alpha Subunits, G12-G13HumansKineticsLuciferasesMolecular Sequence DataMutationPhosphorylationProtein BindingProtein Structure, SecondaryRecombinant ProteinsGTP-Binding Protein alpha Subunits, G12-G13LuciferasesRecombinant ProteinsrhoA GTP-Binding ProteinRho Guanine Nucleotide Exchange FactorsSerineAll-trans retinoic acidATRADbl homologyDHDMEMDulbecco's modified Eagle's mediumFBSFetal bovine serumGalpha13GAPGTPase activating proteinHigh pressure/tandem mass spectrometryHPLC–MS/MSLARGLC/MS/MSLeukemia-associated RhoGEFLiquid chromatography/tandem mass spectrometryLPALysophosphatidic acidNα-p-tosyl-l-lysine phenylalanine chloromethyl ketoneNα-tosyl-l-lysine chloromethyl ketonep115RhoGEFPAR-1PDZPHPhorbol 12-myristate 13-acetatePleckstrin homologyPMAPost-synaptic density protein, discs large protein, zonula occludens-1Protease-activated receptor 1Regulator of G protein signaling homology domain-containing Rho guanine nucleotide exchange factorResponse unitsRhoARH-RhoGEFRUS1PSf9Signal transductionSphingosine-1-phosphateSpodoptera frugiperdaSPRSurface plasmon resonanceTLCKTPCK

Identifiers

PMID23816534
PMCPMC4076829
OpenAlexW1998762736

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.