ArticlePathogens and global health2013
Development and evaluation of a 28S rRNA gene-based nested PCR assay for P. falciparum and P. vivax.
Article in Pathogens and global health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- Differential expression of mitochondria-associated genes in clinical samples of Plasmodium falciparum showing severe manifestations.BMC genomics · 2026Article
- Genome-wide analysis of strand-specific transcription and DNA methylation inFrontiers in microbiology · 2026Article
- Integrative transcriptomic and machine learning approaches to decipher mitochondrial gene regulation in severe Plasmodium vivax malaria.Malaria journal · 2025Article
- A tale of two parasites: a glimpse into the RNA methylome of patient-derived Plasmodium falciparum and Plasmodium vivax isolates.Malaria journal · 2025Article
- Development and Clinical Validation of Iso-IMRS: A Novel Diagnostic Assay forAnalytical chemistry · 2021Article
- Diagnostic Methods for Non-Falciparum Malaria.Frontiers in cellular and infection microbiology · 2021Review
- Reduction of Sphingosine Kinase 1 Phosphorylation and Activity inFrontiers in cell and developmental biology · 2020Article
- A cross strain Plasmodium falciparum microarray optimized for the transcriptome analysis of Plasmodium falciparum patient derived isolates.Genomics data · 2016Article
- Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets.PLoS medicine · 2015Article
- An in vivo transcriptome data set of natural antisense transcripts from Plasmodium falciparum clinical isolates.Genomics data · 2014Article
- Dataset of natural antisense transcripts in P. vivax clinical isolates derived using custom designed strand-specific microarray.Genomics data · 2014Article
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10 authors.
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Abstract
The 28S rRNA gene was amplified and sequenced from P. falciparum and P. vivax isolates collected from northwest India. Based upon the sequence diversity of the Plasmodium 28SrRNA gene in comparison with its human counterpart, various nested polymerase chain reaction (PCR) primers were designed from the 3R region of the 28SrRNA gene and evaluated on field isolates. This is the first report demonstrating the utility of this gene for species-specific diagnosis of malaria for these two species, prevalent in India. The initial evaluation on 363 clinical isolates indicated that, in comparison with microscopy, which showed sensitivity and specificity of 85·39% and 100% respectively, the sensitivity and specificity of the nested PCR assay was found to be 99·08% and 100% respectively. This assay was also successful in detecting mixed infections that are undetected by microscopy. Our results demonstrate the utility of the 28S rRNA gene as a diagnostic target for the detection of the major plasmodial species infecting humans.
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