ArticleGenome biology2013
A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases.
Article in Genome biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish.Science advances · 2021Article
- Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish.Developmental cell · 2019Article
- High Efficiency Targeting of Non-coding Sequences Using CRISPR/Cas9 System in Tilapia.G3 (Bethesda, Md.) · 2019Article
- Analysis of novel domain-specific mutations in the zebrafishJournal of genetics · 2018Article
- LITTLE FISH, BIG DATA: ZEBRAFISH AS A MODEL FOR CARDIOVASCULAR AND METABOLIC DISEASE.Physiological reviews · 2017Review
- Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.Nature reviews. Genetics · 2017Review
- The zebrafish eye-a paradigm for investigating human ocular genetics.Eye (London, England) · 2017Review
- Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system.Cellular and molecular life sciences : CMLS · 2016Article
- Genome-Wide Analysis of Transposon and Retroviral Insertions Reveals Preferential Integrations in Regions of DNA Flexibility.G3 (Bethesda, Md.) · 2016Article
- cncRNAs: Bi-functional RNAs with protein coding and non-coding functions.Seminars in cell & developmental biology · 2015Review
- A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain.Developmental cell · 2015Article
- High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.Genome research · 2015Article
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.Genome research · 2014Article
- Inheritable and precise large genomic deletions of non-coding RNA genes in zebrafish using TALENs.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.