Evidence map›Paper›PMID 23815280›Full record

ArticleACS nano2013

Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules.

Rui Tang, Chang Soo Kim, David J Solfiell, Subinoy Rana, Rubul Mout, Elih M Velázquez-Delgado, Apiwat Chompoosor, Youngdo Jeong, Bo Yan, Zheng-Jiang Zhu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Review
  2. Intracellular delivery of proteins for live cell imaging.Journal of controlled release : official journal of the Controlled Release Society · 2025
    Article
  3. Review
  4. Review
  5. Article
  6. Lagrangian stretching reveals stress topology in viscoelastic flows.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  7. Review
  8. Review
  9. TweenNanomaterials (Basel, Switzerland) · 2021
    Article
  10. Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021
    Review
  11. Stagnation points control chaotic fluctuations in viscoelastic porous media flow.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Article

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Rui TangDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Chang Soo Kim *Department of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
David J Solfiell *Department of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Subinoy Rana *Department of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Rubul MoutDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Elih M Velázquez-DelgadoDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Apiwat ChompoosorDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Youngdo JeongDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Bo YanDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Zheng-Jiang ZhuDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Chaekyu KimDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Jeanne A HardyDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.
Vincent M RotelloDepartment of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, Massachusetts, 01003, USA.

Funding

CHEMISTRY-BIOLOGY INTERFACE PREDOCTORAL TRAINING GRANTST32GM008515 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI THOMPSON, LYNMARIE K. · 1995 to 2020
$4.3M
Control of Executioner Caspases with an allosteric switch - SupplementR01GM080532 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI HARDY, JEANNE ANN · 2008 to 2022
$3.8M
Recognition and Presentation of Alpha Helices using Nanoparticle ReceptorsR01GM077173 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ROTELLO, VINCENT M. · 2007 to 2018
$3.7M
Intracellular Host-Guest Activation of Nanoparticle TherapeuticsR01EB014277 · NIBIB · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ROTELLO, VINCENT M. · 2012 to 2014
$1.0M
NIBIB NIH HHS EB014277NIBIB NIH HHS R01 EB014277NIGMS NIH HHS GM077173NIGMS NIH HHS GM80532NIGMS NIH HHS R01 GM077173NIGMS NIH HHS R01 GM080532NIGMS NIH HHS T32 GM008515PHS HHS S11110000000104
6 · The paper itself

Abstract

Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective protein delivery faces two major challenges: efficient cellular uptake and avoiding endosomal sequestration. We report here a general strategy for direct delivery of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed and stabilized through supramolecular interactions between the nanoparticle, the protein cargo, and the fatty acid capsule interior. The NPSCs are ~130 nm in diameter and feature low toxicity and excellent stability in serum. The effectiveness of these NPSCs as therapeutic protein carriers was demonstrated through the delivery of fully functional caspase-3 to HeLa cells with concomitant apoptosis. Analogous delivery of green fluorescent protein (GFP) confirmed cytosolic delivery as well as intracellular targeting of the delivered protein, demonstrating the utility of the system for both therapeutic and imaging applications.

Indexed as

ApoptosisCaspase 3CytoplasmCytosolDrug CarriersEndosomesFlow CytometryGreen Fluorescent ProteinsHeLa CellsHumansLigandsNanocapsulesNanoparticlesProteinsCaspase 3Drug CarriersGreen Fluorescent ProteinsLigandsNanocapsulesProteins

Identifiers

PMID23815280
PMCPMC3757120

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.