ArticleNucleic acids research2013
Carcinogenic adducts induce distinct DNA polymerase binding orientations.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
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- Bulky Lesion Bypass Requires Dpo4 Binding in Distinct Conformations.Scientific reports · 2017Article
- Advances in Structural and Single-Molecule Methods for Investigating DNA Lesion Bypass and Repair Polymerases.Chemical research in toxicology · 2017Article
- Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers.Scientific reports · 2016Article
- Single Molecule Bioelectronics and Their Application to Amplification-Free Measurement of DNA Lengths.Biosensors · 2016Article
- Mechanism of aromatic amine carcinogen bypass by the Y-family polymerase, Dpo4.Nucleic acids research · 2015Article
- Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms.Nucleic acids research · 2015Article
- Real-time surface plasmon resonance study of biomolecular interactions between polymerase and bulky mutagenic DNA lesions.Chemical research in toxicology · 2014Article
- Mechanisms of mutagenesis: DNA replication in the presence of DNA damage.Mutation research. Reviews in mutation researchReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DNA polymerases must accurately replicate DNA to maintain genome integrity. Carcinogenic adducts, such as 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF), covalently bind DNA bases and promote mutagenesis near the adduct site. The mechanism by which carcinogenic adducts inhibit DNA synthesis and cause mutagenesis remains unclear. Here, we measure interactions between a DNA polymerase and carcinogenic DNA adducts in real-time by single-molecule fluorescence. We find the degree to which an adduct affects polymerase binding to the DNA depends on the adduct location with respect to the primer terminus, the adduct structure and the nucleotides present in the solution. Not only do the adducts influence the polymerase dwell time on the DNA but also its binding position and orientation. Finally, we have directly observed an adduct- and mismatch-induced intermediate state, which may be an obligatory step in the DNA polymerase proofreading mechanism.
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Registered trials
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