ArticleNucleic acids research2013
Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Recent progress in single-molecule studies of mRNA localizationRNA biology · 2019Review
- Nucleic Acid Strand Displacement with Synthetic mRNA Inputs in Living Mammalian Cells.ACS synthetic biology · 2018Article
- Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.Nucleic acids research · 2018Article
- A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.Scientific reports · 2017Article
- Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.Stem cell reports · 2015Article
- DNA nanotechnology from the test tube to the cell.Nature nanotechnology · 2015Review
- Molecular beacon-based detection and isolation of working-type cardiomyocytes derived from human pluripotent stem cells.Biomaterials · 2015Article
- Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons.Journal of visualized experiments : JoVE · 2014Article
- Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons.PloS one · 2014Article
- Inside single cells: quantitative analysis with advanced optics and nanomaterials.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
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Authors and funding
8 authors.
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Abstract
Recently, we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent signal in living cells that express the target RNA. Here, we show that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is engineered to contain as few as four hybridization sites. Moreover, comparison with single-molecule fluorescence in situ hybridization confirmed that RBMBs could be used to accurately quantify the number of RNA transcripts within individual cells. Measurements of gene expression could be acquired within 30 min and using a wide range of RBMB concentrations. The ability to acquire accurate measurements of RNA copy number in both HT-1080 cells and CHO cells also suggests that RBMBs can be used to image and quantify single RNA transcripts in a wide range of cell lines. Overall, these findings highlight the robustness and versatility of RBMBs as a tool for imaging RNA in live cells. We envision that the unique capabilities of RBMBs will open up new avenues for RNA research.
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