ArticleArteriosclerosis, thrombosis, and vascular biology2013
Endothelial cell sensing of flow direction.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02982044 (Endothelial Function Responses to Manipulations in Blood Flow Pattern), which is not on this map. Cited by 162 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Endothelial Function Responses to Manipulations in Blood Flow Pattern
Who cites it
162 citing papers in PubMed, 1 synthesis or guideline pooled it, 286 citations in OpenAlex.
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- Hemodynamic Metrics and Arterial Dysfunction: Insights From a Novel Microfluidic Device.International journal for numerical methods in biomedical engineering · 2026Article
- Decoding the causal drivers of spatial cellular topology.iScience · 2026Article
- Shear cytokine crosstalk is a determinant of SARS-CoV-2-induced endothelial pathophysiology and thrombosis in human vessel chips.Journal of thrombosis and haemostasis : JTH · 2026Article
- Automating Vascular Biology: An End-to-End Automated Workflow for High-Throughput Blood Vessel-on-a-Chip Production and Multi-Site Validation.Advanced healthcare materials · 2026Article
- Next Generation Experimental Models of Venous Physiology and Pathology: Examining Human Relevance in Recapitulating Lower Extremity Flow and Valve Function in New Approach Methods (NAMs).Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Endothelial Nuclear and Cytoskeletal Responses to Mechanical Stresses.Advances in experimental medicine and biology · 2026Article
- Active endothelial and neural regulation of valve biology, health, and disease.Frontiers in cardiovascular medicine · 2026Review
- A Patient-Specific 3D Printed Carotid Artery Model Integrating Vascular Structure, Flow, and Endothelium Responses.Advanced healthcare materials · 2026Article
- Vascular endothelial integration of multiple biophysical stimuli.Frontiers in cardiovascular medicine · 2026Article
- A cell and transcriptome atlas of human arterial vasculature.Cell genomics · 2025Article
- Whole-body hot water immersion effect on cerebral haemodynamics and subsequent cerebrovascular reactivity to carbon dioxide.Experimental physiology · 2025Article
- Hemodynamics in carotid artery stenosis.International angiology : a journal of the International Union of Angiology · 2025Review
- Effects of repeated sprint exercise on brachial artery shear rate patterns in healthy adults.Physiological reports · 2025Article
- Oversized Conduits Predict Stenosis in Tissue Engineered Vascular Grafts.JACC. Basic to translational science · 2025Article
- Multidimensional excavation of the current status and trends of mechanobiology in cardiovascular homeostasis and remodeling within 20 years.Mechanobiology in medicine · 2025Review
102 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectiveAtherosclerosis-prone regions of arteries are characterized by complex flow patterns where the magnitude of shear stress is low and direction rapidly changes, termed disturbed flow. How endothelial cells sense flow direction and how it impacts inflammatory effects of disturbed flow are unknown. We therefore aimed to understand how endothelial cells respond to changes in flow direction. APPROACH AND
resultsUsing a recently developed flow system capable of changing flow direction to any angle, we show that responses of aligned endothelial cells are determined by flow direction relative to their morphological and cytoskeletal axis. Activation of the atheroprotective endothelial nitric oxide synthase pathway is maximal at 180° and undetectable at 90°, whereas activation of proinflammatory nuclear factor-κB is maximal at 90° and undetectable at 180°. Similar effects were observed in randomly oriented cells in naive monolayers subjected to onset of shear. Cells aligned on micropatterned substrates subjected to oscillatory flow were also examined. In this system, parallel flow preferentially activated endothelial nitric oxide synthase and production of nitric oxide, whereas perpendicular flow preferentially activated reactive oxygen production and nuclear factor-κB.
conclusionsThese data show that the angle between flow and the cell axis defined by their shape and cytoskeleton determines endothelial cell responses. The data also strongly suggest that the inability of cells to align in low and oscillatory flow is a key determinant of the resultant inflammatory activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.