ArticleThe Journal of biological chemistry2013
Radiolytic mapping of solvent-contact surfaces in Photosystem II of higher plants: experimental identification of putative water channels within the photosystem.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Probing substrate water access through the O1 channel of Photosystem II by single site mutations and membrane inlet mass spectrometry.Photosynthesis research · 2025Article
- Structure Function Studies of Photosystem II Using X-Ray Free Electron Lasers.Annual review of biophysics · 2024Review
- Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography.IUCrJ · 2023Article
- Advances in Mass Spectrometry on Membrane Proteins.Membranes · 2023Review
- Oxidative modification of LHC II associated with photosystem II and PS I-LHC I-LHC II membranes.Photosynthesis research · 2022Article
- MEMBRANE PROTEIN STRUCTURES AND INTERACTIONS FROM COVALENT LABELING COUPLED WITH MASS SPECTROMETRY.Mass spectrometry reviews · 2022Review
- Photosystem II oxygen-evolving complex photoassembly displays an inverse H/D solvent isotope effect under chloride-limiting conditions.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Reactive oxygen species leave a damage trail that reveals water channels in Photosystem II.Science advances · 2017Article
- Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Insights into Photosystem II from Isomorphous Difference Fourier Maps of Femtosecond X-ray Diffraction Data and Quantum Mechanics/Molecular Mechanics Structural Models.ACS energy letters · 2017Review
- Synchrotron X-ray footprinting as a method to visualize water in proteins.Journal of synchrotron radiation · 2016Article
- X-ray radiation-induced addition of oxygen atoms to protein residues.Protein science : a publication of the Protein Society · 2016Article
- The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.Frontiers in plant science · 2016Review
- Local and global structural drivers for the photoactivation of the orange carotenoid protein.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Use of protein cross-linking and radiolytic footprinting to elucidate PsbP and PsbQ interactions within higher plant Photosystem II.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Photosystem II uses water as an enzymatic substrate. It has been hypothesized that this water is vectored to the active site for water oxidation via water channels that lead from the surface of the protein complex to the Mn4O5Ca metal cluster. The radiolysis of water by synchrotron radiation produces amino acid residue-modifying OH(•) and is a powerful technique to identify regions of proteins that are in contact with water. In this study, we have used this technique to oxidatively modify buried amino acid residues in higher plant Photosystem II membranes. Fourier transform ion cyclotron resonance mass spectrometry was then used to identify these oxidized amino acid residues that were located in several core Photosystem II subunits (D1, D2, CP43, and CP47). While, as expected, the majority of the identified oxidized residues (≈75%) are located on the solvent-exposed surface of the complex, a number of buried residues on these proteins were also modified. These residues form groups which appear to lead from the surface of the complex to the Mn4O5Ca cluster. These residues may be in contact with putative water channels in the photosystem. These results are discussed within the context of a number of largely computational studies that have identified putative water channels in Photosystem II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.