ArticleJournal of bacteriology2013
Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging.Nature methods · 2026Article
- Polyphosphate: The "Dark Matter" of Bacterial Chromatin Structure.Molecular microbiology · 2025Review
- Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.Nature methods · 2024Article
- Reentrant DNA shells tune polyphosphate condensate size.Nature communications · 2024Article
- Quantitative Analysis ofBiomolecules · 2024Article
- A widespread bacterial protein compartment sequesters and stores elemental sulfur.Science advances · 2024Article
- Ultrastructural insights into cellular organization, energy storage and ribosomal dynamics of an ammonia-oxidizing archaeon from oligotrophic oceans.Frontiers in microbiology · 2024Article
- Cryo-electron tomography of stationary phasemicroPublication biology · 2024Article
- Reentrant DNA shells tune polyphosphate condensate size.bioRxiv : the preprint server for biology · 2023Article
- Article
- Super-resolution confocal cryo-CLEM with cryo-FIB milling forCurrent research in structural biology · 2022Article
- Article
- Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.Journal of thrombosis and haemostasis : JTH · 2020Article
- Polyphosphate FunctionsmBio · 2020Article
- Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Over Winter Microbial Processes in a Svalbard Snow Pack: An Experimental Approach.Frontiers in microbiology · 2020Article
- Biopearling of Interconnected Outer Membrane Vesicle Chains by a Marine Flavobacterium.Applied and environmental microbiology · 2019Article
- A Heat Shock Protein 48 (HSP48) Biomolecular Condensate Is Induced during Dictyostelium discoideum Development.mSphere · 2019Article
- Biogenic Polyphosphate Nanoparticles from a Marine CyanobacteriumMarine drugs · 2018Article
- Novel Mechanism for Surface Layer Shedding and Regenerating in Bacteria Exposed to Metal-Contaminated Conditions.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Using electron cryotomography, we show that the Gram-negative sporulating bacterium Acetonema longum synthesizes high-density storage granules at the leading edges of engulfing membranes. The granules appear in the prespore and increase in size and number as engulfment proceeds. Typically, a cluster of 8 to 12 storage granules closely associates with the inner spore membrane and ultimately accounts for ∼7% of the total volume in mature spores. Energy-dispersive X-ray spectroscopy (EDX) analyses show that the granules contain high levels of phosphorus, oxygen, and magnesium and therefore are likely composed of polyphosphate (poly-P). Unlike the Gram-positive Bacilli and Clostridia, A. longum spores retain their outer spore membrane upon germination. To explore the possibility that the granules in A. longum may be involved in this unique process, we imaged purified Bacillus cereus, Bacillus thuringiensis, Bacillus subtilis, and Clostridium sporogenes spores. Even though B. cereus and B. thuringiensis contain the ppk and ppx genes, none of the spores from Gram-positive bacteria had granules. We speculate that poly-P in A. longum may provide either the energy or phosphate metabolites needed for outgrowth while retaining an outer membrane.
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