ArticleMicrobiology (Reading, England)2013
Reversible acetylation regulates acetate and propionate metabolism in Mycobacterium smegmatis.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Integrating chemical, genetic, and feasibility assessments for anti-tubercular target validation.EMBO molecular medicine · 2026Review
- Effect of TetR Family Transcriptional Regulator PccD on Phytosterol Metabolism ofMicroorganisms · 2024Article
- Bacterial protein acetylation: mechanisms, functions, and methods for study.Frontiers in cellular and infection microbiology · 2024Review
- Negative regulation of the acsA1 gene encoding the major acetyl-CoA synthetase by cAMP receptor protein in Mycobacterium smegmatis.Journal of microbiology (Seoul, Korea) · 2022Article
- Evidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex.Journal of bacteriology · 2021Article
- Article
- Bacterial Sirtuins Overview: An Open Niche to Explore.Frontiers in microbiology · 2021Review
- Genome-Wide Analyses of Proteome and Acetylome inFrontiers in microbiology · 2021Article
- Post-translational Succinylation ofACS infectious diseases · 2020Article
- Deacetylation enhances ParB-DNA interactions affecting chromosome segregation in Streptomyces coelicolor.Nucleic acids research · 2020Article
- A universal stress protein inThe Journal of biological chemistry · 2020Article
- The Nitrogen Regulator GlnR Directly Controls Transcription of theJournal of bacteriology · 2019Article
- Post-translational Protein Acetylation: An Elegant Mechanism for Bacteria to Dynamically Regulate Metabolic Functions.Frontiers in microbiology · 2019Review
- Cyclic nucleotide signaling in Mycobacterium tuberculosis: an expanding repertoire.Pathogens and disease · 2018Article
- More than cholesterol catabolism: regulatory vulnerabilities in Mycobacterium tuberculosis.Current opinion in chemical biology · 2018Review
- GlnR-Mediated Regulation of Short-Chain Fatty Acid Assimilation inFrontiers in microbiology · 2018Article
- Global Profiling of Lysine Acetylation inFrontiers in microbiology · 2018Article
- NJournal of proteome research · 2017Article
- Regulation, Function, and Detection of Protein Acetylation in Bacteria.Journal of bacteriology · 2017Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Carbon metabolic pathways are important to the pathogenesis of Mycobacterium tuberculosis, the causative agent of tuberculosis. However, extremely little is known about metabolic regulation in mycobacteria. There is growing evidence for lysine acetylation being a mechanism of regulating bacterial metabolism. Lysine acetylation is a post-translational modification in which an acetyl group is covalently attached to the side chain of a lysine residue. This modification is mediated by acetyltransferases, which add acetyl groups, and deacetylases, which remove the acetyl groups. Here we set out to test whether lysine acetylation and deacetylation impact acetate metabolism in the model mycobacteria Mycobacterium smegmatis, which possesses 25 candidate acetyltransferases and 3 putative lysine deacetylases. Using mutants lacking predicted acetyltransferases and deacetylases we showed that acetate metabolism in M. smegmatis is regulated by reversible acetylation of acetyl-CoA synthetase (Ms-Acs) through the action of a single pair of enzymes: the acetyltransferase Ms-PatA and the sirtuin deacetylase Ms-SrtN. We also confirmed that the role of Ms-PatA in regulating Ms-Acs regulation depends on cAMP binding. We additionally demonstrated a role for Ms-Acs, Ms-PatA and Ms-SrtN in regulating the metabolism of propionate in M. smegmatis. Finally, along with Ms-Acs, we identified a candidate propionyl-CoA synthetase, Ms5404, as acetylated in whole-cell lysates. This work lays the foundation for studying the regulatory circuit of acetylation and deacetylation in the cellular context of mycobacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.