ArticleMolecular genetics and genomics : MGG2013
RNA editing events in mitochondrial genes by ultra-deep sequencing methods: a comparison of cytoplasmic male sterile, fertile and restored genotypes in cotton.
Article in Molecular genetics and genomics : MGG, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Mitochondrial-Nuclear Interactions, Co-Transcription, and Adaptive Evolution in Cytoplasmic Male Sterility.Plant, cell & environment · 2026Review
- Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.The plant genome · 2024Article
- Mitochondrial genome ofFrontiers in plant science · 2024Article
- A super PPR cluster for restoring fertility revealed by genetic mapping, homocap-seq and de novo assembly in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Mitochondrial gene expression analysis reveals aberrant transcription of cox3 in Gossypium barbadense CMS line H276A.Development genes and evolution · 2022Article
- The Gossypium anomalum genome as a resource for cotton improvement and evolutionary analysis of hybrid incompatibility.G3 (Bethesda, Md.) · 2021Article
- Comparative RNA editing profile of mitochondrial transcripts in cytoplasmic male sterile and fertile pigeonpea reveal significant changes at the protein level.Molecular biology reports · 2019Article
- RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A.Biological research · 2019Article
- Article
- Genomewide identification of PPR gene family and prediction analysis on restorer gene inJournal of genetics · 2018Article
- Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton.BMC genomics · 2018Article
- Rapid evolutionary divergence of diploid and allotetraploid Gossypium mitochondrial genomes.BMC genomics · 2017Article
- Whole plastid transcriptomes reveal abundant RNA editing sites and differential editing status in Phalaenopsis aphrodite subsp. formosana.Botanical studies · 2017Article
- Genome-wide comparative transcriptome analysis of CMS-D2 and its maintainer and restorer lines in upland cotton.BMC genomics · 2017Article
- Article
- Towards an improved apple reference transcriptome using RNA-seq.Molecular genetics and genomics : MGG · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Cytoplasmic male sterility (CMS) is a maternally inherited trait resulting in failure to produce functional pollen and is widely used in the production of hybrid seed. Improper RNA editing is implicated as the molecular basis for some CMS systems. However, the mechanism of CMS in cotton is unknown. This study compared RNA editing events in eight mitochondrial genes (atp1, 4, 6, 8, 9, and cox1, 2, 3) among three lines (maintainer B, CMS A, and restorer R). These events were quantified by ultra-deep sequencing of mitochondrial transcripts and sequencing of cloned versions of these genes as cDNAs. A comparison of genomic PCR and RT-PCR products detected 72 editing sites in coding sequences in the eight genes and four partial editing sites in the 3'-untranslated region of atp6. The most frequent alteration (61.4 %) resulted in changes of hydrophilic amino acids to hydrophobic amino acids and the most common alteration was proline (P) to leucine (L) (26.7 %). In atp6, RNA editing created a stop codon from a glutamine in the genomic sequence. Statistical analysis of the frequencies of RNA editing events detected differences between mtDNA genes, but no differences between cotton cytoplasms that could account for the CMS phenotype or restoration. This study represents the first work to use next-generation sequencing to identify RNA editing positions and efficiency, and possible association with CMS and restoration in plants.
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Registered trials
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