ArticleObstetrics and gynecology2013
Global gene expression analysis of term amniotic fluid cell-free fetal RNA.
Article in Obstetrics and gynecology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Identification of gestational-age-dependent cell-free fetal mRNA transcriptional changes in human amniotic fluid.Journal of human genetics · 2026Article
- Evaluating the Utility of RNAseq in Prenatal Diagnostics: Expression Profiles of Cultured Chorionic Villus and Amniotic Fluid Samples.Prenatal diagnosis · 2026Article
- Amniotic Fluid Extracellular Vesicle Properties Evolve With Gestational Age and Reflect Fetal Development.Journal of extracellular biology · 2025Article
- The impact of AMniotic FluId on the development and microBIal colonization of the prEterm intestinal tract (AMFIBIE): study protocol for a multicenter prospective cohort study.Frontiers in pediatrics · 2025Article
- The amniotic fluid proteome changes with term labor and informs biomarker discovery in maternal plasma.Scientific reports · 2023Article
- Emerging technologies for prenatal diagnosis: The application of whole genome and RNA sequencing.Prenatal diagnosis · 2022Review
- The amniotic fluid proteome changes with gestational age in normal pregnancy: a cross-sectional study.Scientific reports · 2022Article
- MicroRNAs in amniotic fluid and maternal blood plasma associated with sex determination and early gonad differentiation in cattle†.Biology of reproduction · 2021Article
- Transcriptomic Analysis of Cell-free Fetal RNA in the Amniotic Fluid of Vervet Monkeys (Comparative medicine · 2020Article
- RNA-Sequencing of Umbilical Cord Blood to Investigate Spontaneous Preterm Birth: A Pilot Study.AJP reports · 2019Article
- Next-generation sequencing and prenatal 'omics: advanced diagnostics and new insights into human development.Genetics in medicine : official journal of the American College of Medical Genetics · 2018Review
- Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction.Medicine · 2018Article
- An Integrated Human/Murine Transcriptome and Pathway Approach To Identify Prenatal Treatments For Down Syndrome.Scientific reports · 2016Article
- A comparison of sample collection methods for quantifying cell-free fetal neurodevelopment transcripts in amniotic fluid.BMC research notes · 2016Article
- microRNA Profiling of Amniotic Fluid: Evidence of Synergy of microRNAs in Fetal Development.PloS one · 2016Article
- Systems biology evaluation of cell-free amniotic fluid transcriptome of term and preterm infants to detect fetal maturity.BMC medical genomics · 2015Article
- Effects of Maternal Obesity on Fetal Programming: Molecular Approaches.Cold Spring Harbor perspectives in medicine · 2015Review
- The pathway not taken: understanding 'omics data in the perinatal context.American journal of obstetrics and gynecology · 2015Article
- The amniotic fluid transcriptome as a guide to understanding fetal disease.Cold Spring Harbor perspectives in medicine · 2015Review
- Comparative Transcriptome Analysis of Cell-Free Fetal RNA from Amniotic Fluid and RNA from Amniocytes in Uncomplicated Pregnancies.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo identify the tissue expression patterns and biological pathways enriched in term amniotic fluid cell-free fetal RNA by comparing functional genomic analyses of term and second-trimester amniotic fluid supernatants.
methodsThis was a prospective whole genome microarray study comparing eight amniotic fluid samples collected from women at term who underwent prelabor cesarean delivery and eight second-trimester amniotic fluid samples from routine amniocenteses. A functional annotation tool was used to compare tissue expression patterns in term and second-trimester samples. Pathways analysis software identified physiologic systems, molecular and cellular functions, and upstream regulators that were significantly overrepresented in term amniotic fluid.
resultsThere were 2,871 significantly differentially regulated genes. In term amniotic fluid, tissue expression analysis showed enrichment of salivary gland, tracheal, and renal transcripts as compared with brain and embryonic neural cells in the second trimester. Functional analysis of genes upregulated at term revealed pathways that were highly specific for postnatal adaptation such as immune function, digestion, respiration, carbohydrate metabolism, and adipogenesis. Inflammation and prostaglandin synthesis, two key processes involved in normal labor, were also activated in term amniotic fluid.
conclusionsTranscriptomic analysis of amniotic fluid cell-free fetal RNA detects fetal maturation processes activated in term pregnancy. These findings further develop the concept of amniotic fluid supernatant as a real-time gene expression "summary fluid" and support its potential for future studies of fetal development.
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