ArticleCardiovascular research2013
Blood flow suppresses vascular Notch signalling via dll4 and is required for angiogenesis in response to hypoxic signalling.
Article in Cardiovascular research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Review
- RNA-seq analysis of blood from cave- and surface-dwellingFrontiers in physiology · 2025Article
- Zebrafish model for functional screening of flow-responsive genes controlling endothelial cell proliferation.Scientific reports · 2024Article
- Plxnd1-mediated mechanosensing of blood flow controls the caliber of the Dorsal Aorta via the transcription factor Klf2.bioRxiv : the preprint server for biology · 2024Article
- The conserved wobble uridine tRNA thiolase Ctu1 is required for angiogenesis and embryonic development.PloS one · 2024Article
- Zebrafish vascular quantification: a tool for quantification of three-dimensional zebrafish cerebrovascular architecture by automated image analysis.Development (Cambridge, England) · 2022Article
- The effect of absent blood flow on the zebrafish cerebral and trunk vasculature.Vascular biology (Bristol, England) · 2021Article
- Caveolin‑1 modulates hypertensive vascular remodeling via regulation of the Notch pathway.Molecular medicine reports · 2020Article
- Endothelial responses to shear stress in atherosclerosis: a novel role for developmental genes.Nature reviews. Cardiology · 2020Review
- Artery-vein specification in the zebrafish trunk is pre-patterned by heterogeneous Notch activity and balanced by flow-mediated fine-tuning.Development (Cambridge, England) · 2019Article
- Cerebrovascular endothelial cells form transient Notch-dependent cystic structures in zebrafish.EMBO reports · 2019Article
- Blood flow-induced Notch activation and endothelial migration enable vascular remodeling in zebrafish embryos.Nature communications · 2018Article
- Cancer cell adaptation to hypoxia involves a HIF-GPRC5A-YAP axis.EMBO molecular medicine · 2018Article
- Mechanosensitivity of Jagged-Notch signaling can induce a switch-type behavior in vascular homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Flow Induced Microvascular Network Formation of Therapeutic Relevant Arteriovenous (AV) Loop-Based Constructs in Response to Ionizing Radiation.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- DLL4 overexpression increases gastric cancer stem/progenitor cell self-renewal ability and correlates with poor clinical outcome via Notch-1 signaling pathway activation.Cancer medicine · 2017Article
- Blood flow controls bone vascular function and osteogenesis.Nature communications · 2016Article
- Detection of initial angiogenesis from dorsal aorta into metanephroi and elucidation of its role in kidney development.Regenerative therapy · 2016Article
- Reck enables cerebrovascular development by promoting canonical Wnt signaling.Development (Cambridge, England) · 2016Article
- Exploring the HIFs, buts and maybes of hypoxia signalling in disease: lessons from zebrafish models.Disease models & mechanisms · 2015Review
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
aimsThe contribution of blood flow to angiogenesis is incompletely understood. We examined the effect of blood flow on Notch signalling in the vasculature of zebrafish embryos, and whether blood flow regulates angiogenesis in zebrafish with constitutively up-regulated hypoxic signalling. METHODS AND
resultsDeveloping zebrafish (Danio rerio) embryos survive via diffusion in the absence of circulation induced by knockdown of cardiac troponin T2 or chemical cardiac cessation. The absence of blood flow increased vascular Notch signalling in 48 h post-fertilization old embryos via up-regulation of the Notch ligand dll4. Despite this, patterning of the intersegmental vessels is not affected by absent blood flow. We therefore examined homozygous vhl mutant zebrafish that have constitutively up-regulated hypoxic signalling. These display excessive and aberrant angiogenesis from 72 h post-fertilization, with significantly increased endothelial number, vessel diameter, and length. The absence of blood flow abolished these effects, though normal vessel patterning was preserved.
conclusionWe show that blood flow suppresses vascular Notch signalling via down-regulation of dll4. We have also shown that blood flow is required for angiogenesis in response to hypoxic signalling but is not required for normal vessel patterning. These data indicate important differences in hypoxia-driven vs. developmental angiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.