ArticleInternational journal of environmental research and public health2013
Detection of β-lactamase residues in milk by sandwich ELISA.
Article in International journal of environmental research and public health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 25 citations in OpenAlex.
- Detection of Antimicrobial Proteins/Peptides and Bacterial Proteins Involved in Antimicrobial Resistance in Raw Cow's Milk from Different Breeds.Antibiotics (Basel, Switzerland) · 2024Article
- Immunochemical assay with monoclonal antibodies for detection of staphylococcal enterotoxin H.Journal of food and drug analysis · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
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Abstract
β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5-100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82-103.13%. The intra- and inter-assay coefficients of variation were 6.21-7.38% and 12.96-13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk.
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