ArticleApplied and environmental microbiology2013
Fermentation temperature modulates phosphatidylethanolamine and phosphatidylinositol levels in the cell membrane of Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 44 citations in OpenAlex.
- De novo genome assembly of a native Saccharomyces cerevisiae strain isolated from spontaneous fermentation of vineyard must in the Atacama Desert (Chile).FEMS yeast research · 2026Article
- Microbiome-mediated flavor development in Berbassa: a traditional fermented milk starter for Gergoush production.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Comparison of stress tolerance mechanisms between Saccharomyces cerevisiae and the multistress-tolerant Pichia kudriavzevii.FEMS yeast research · 2025Review
- Human Sterols Are Overproduced, Stored and Excreted in Yeasts.International journal of molecular sciences · 2024Article
- Downshifting Yeast Dominance: Cell Physiology and Phospholipid Composition Are Altered With Establishment of the [Frontiers in microbiology · 2020Article
- SLIMEr: probing flexibility of lipid metabolism in yeast with an improved constraint-based modeling framework.BMC systems biology · 2019Article
- Genetic Basis of Variation in Heat and Ethanol Tolerance inG3 (Bethesda, Md.) · 2019Article
- Construction of industrialBiotechnology for biofuels · 2019Article
- High-level recombinant production of squalene using selected Saccharomyces cerevisiae strains.Journal of industrial microbiology & biotechnology · 2018Article
- Inheritance of brewing-relevant phenotypes in constructed Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids.Microbial cell factories · 2017Article
- Four Acyltransferases Uniquely Contribute to Phospholipid Heterogeneity inLipid insights · 2016Article
- Article
- Physiological and transcriptional responses of anaerobic chemostat cultures of Saccharomyces cerevisiae subjected to diurnal temperature cycles.Applied and environmental microbiology · 2014Article
- Examining the role of membrane lipid composition in determining the ethanol tolerance of Saccharomyces cerevisiae.Applied and environmental microbiology · 2014Article
- Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
During alcoholic fermentation, Saccharomyces cerevisiae is exposed to a host of environmental and physiological stresses. Extremes of fermentation temperature have previously been demonstrated to induce fermentation arrest under growth conditions that would otherwise result in complete sugar utilization at "normal" temperatures and nutrient levels. Fermentations were carried out at 15°C, 25°C, and 35°C in a defined high-sugar medium using three Saccharomyces cerevisiae strains with diverse fermentation characteristics. The lipid composition of these strains was analyzed at two fermentation stages, when ethanol levels were low early in stationary phase and in late stationary phase at high ethanol concentrations. Several lipids exhibited dramatic differences in membrane concentration in a temperature-dependent manner. Principal component analysis (PCA) was used as a tool to elucidate correlations between specific lipid species and fermentation temperature for each yeast strain. Fermentations carried out at 35°C exhibited very high concentrations of several phosphatidylinositol species, whereas at 15°C these yeast strains exhibited higher levels of phosphatidylethanolamine and phosphatidylcholine species with medium-chain fatty acids. Furthermore, membrane concentrations of ergosterol were highest in the yeast strain that experienced stuck fermentations at all three temperatures. Fluorescence anisotropy measurements of yeast cell membrane fluidity during fermentation were carried out using the lipophilic fluorophore diphenylhexatriene. These measurements demonstrate that the changes in the lipid composition of these yeast strains across the range of fermentation temperatures used in this study did not significantly affect cell membrane fluidity. However, the results from this study indicate that fermenting S. cerevisiae modulates its membrane lipid composition in a temperature-dependent manner.
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