Evidence map›Paper›PMID 23811515›Full record

ArticleApplied and environmental microbiology2013

Host association of Cryptosporidium parvum populations infecting domestic ruminants in Spain.

Joaquín Quílez, Claudia Vergara-Castiblanco, Luis Monteagudo, Emilio del Cacho, Caridad Sánchez-Acedo

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Molecular analysis of cryptosporidiosis on cattle farms in Gran Canaria, Canary Islands (Spain).International journal of veterinary science and medicine · 2025
    Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Direct Sequencing ofFrontiers in public health · 2019
    Review
  9. Alterations in oxidative stress parameters and its associated correlation with clinical disease on experimentalJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017
    Article
  10. Gastroenterology and hepatology from bed to bench · 2017
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joaquín QuílezDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain. jquilez@unizar.es
Claudia Vergara-Castiblanco
Luis Monteagudo
Emilio del Cacho
Caridad Sánchez-Acedo

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A stock of 148 Cryptosporidium parvum DNA extracts from lambs and goat kids selected from a previous study examining the occurrence of Cryptosporidium species and GP60 subtypes in diarrheic lambs and goat kids in northeastern Spain was further characterized by a multilocus fragment typing approach with six mini- and microsatellite loci. Various degrees of polymorphism were seen at all but the MS5 locus, although all markers exhibited two major alleles accounting for more than 75% of isolates. A total of 56 multilocus subtypes (MLTs) from lambs (48 MLTs) and goat kids (11 MLTs) were identified. Individual isolates with mixed MLTs were detected on more than 25% of the farms, but most MLTs (33) were distinctive for individual farms, revealing the endemicity of cryptosporidial infections on sheep and goat farms. Comparison with a previous study in calves in northern Spain using the same six-locus subtyping scheme showed the presence of host-associated alleles, differences in the identity of major alleles, and very little overlap in MLTs between C. parvum isolates from lambs and those from calves (1 MLT) or isolates from lambs and those from goat kids (3 MLTs). The Hunter-Gaston index of the multilocus technique was 0.976 (95% confidence interval [CI], 0.970 to 0.982), which supports its high discriminatory power for strain typing and epidemiological tracking. Population analyses revealed the presence of two host-associated subpopulations showing epidemic clonality among the C. parvum isolates infecting calves and lambs/goat kids, respectively, although evidence of genetic flow between the two subpopulations was also detected.

Indexed as

Genetic VariationAnimalsCryptosporidiosisCryptosporidium parvumEndemic DiseasesGenotypeGoat DiseasesGoatsMicrosatellite RepeatsMolecular EpidemiologyMolecular Sequence DataMolecular TypingMycological Typing TechniquesSequence Analysis, DNASheepSheep Diseases

Identifiers

PMID23811515
PMCPMC3753963

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.