ArticleApplied and environmental microbiology2013
Genetic determinants for n-butanol tolerance in evolved Escherichia coli mutants: cross adaptation and antagonistic pleiotropy between n-butanol and other stressors.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 57 citations in OpenAlex.
- Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.International journal of molecular sciences · 2024Article
- Correlated responses to selection across diverse environments during experimental evolution ofEcology and evolution · 2024Article
- Pleiotropy alleviates the fitness costs associated with resource allocation trade-offs in immune signalling networks.Proceedings. Biological sciences · 2024Article
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Pleiotropy alleviates the fitness costs associated with resource allocation trade-offs in immune signaling networks.bioRxiv : the preprint server for biology · 2023Article
- Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation.Applied microbiology and biotechnology · 2021Article
- Genomics and transcriptomics analysis reveals the mechanism of isobutanol tolerance of a laboratory evolved Lactococcus lactis strain.Scientific reports · 2020Article
- Understanding metabolic adaptation by using bacterial laboratory evolution and trans-omics analysis.Biophysical reviews · 2020Review
- Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Continuous Culture Adaptation ofFrontiers in microbiology · 2019Article
- Identification of functional butanol-tolerant genes fromBiotechnology for biofuels · 2019Article
- Understanding and engineering alcohol-tolerant bacteria using OMICS technology.World journal of microbiology & biotechnology · 2018Review
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance.Frontiers in bioengineering and biotechnology · 2018Article
- A New Player in the Biorefineries Field: Phasin PhaP Enhances Tolerance to Solvents and Boosts Ethanol and 1,3-Propanediol Synthesis in Escherichia coli.Applied and environmental microbiology · 2017Article
- Progress and perspectives on improving butanol tolerance.World journal of microbiology & biotechnology · 2017Review
- Genome-wide Escherichia coli stress response and improved tolerance towards industrially relevant chemicals.Microbial cell factories · 2016Article
- Engineering membrane and cell-wall programs for tolerance to toxic chemicals: Beyond solo genes.Current opinion in microbiology · 2016Review
- Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.BMC microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cross-tolerance and antagonistic pleiotropy have been observed between different complex phenotypes in microbial systems. These relationships between adaptive landscapes are important for the design of industrially relevant strains, which are generally subjected to multiple stressors. In our previous work, we evolved Escherichia coli for enhanced tolerance to the biofuel n-butanol and discovered a molecular mechanism of n-butanol tolerance that also conferred tolerance to the cationic antimicrobial peptide polymyxin B in one specific lineage (green fluorescent protein [GFP] labeled) in the evolved population. In this work, we aim to identify additional mechanisms of n-butanol tolerance in an independent lineage (yellow fluorescent protein [YFP] labeled) from the same evolved population and to further explore potential cross-tolerance and antagonistic pleiotropy between n-butanol tolerance and other industrially relevant stressors. Analysis of the transcriptome data of the YFP-labeled mutants allowed us to discover additional membrane-related and osmotic stress-related genes that confer n-butanol tolerance in E. coli. Interestingly, the n-butanol resistance mechanisms conferred by the membrane-related genes appear to be specific to n-butanol and are in many cases antagonistic with isobutanol and ethanol. Furthermore, the YFP-labeled mutants showed cross-tolerance between n-butanol and osmotic stress, while the GFP-labeled mutants showed antagonistic pleiotropy between n-butanol and osmotic stress tolerance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.