Evidence map›Paper›PMID 23810829›Full record

ArticleNeuropharmacology2013

Intrathecal P/Q- and R-type calcium channel blockade of spinal substance P release and c-Fos expression.

Tetsuji Terashima, Qinghao Xu, Shigeki Yamaguchi, Tony L Yaksh

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Tetsuji TerashimaDepartment of Anesthesiology, Dokkyo Medical University, School of Medicine, Kitakobayashi 880, Mibu, Tochigi 321-0293, Japan; Department of Anesthesiology, University of California, San Diego, 9500 Gilman Drive, San Diego, La Jolla, CA 92093-0818, USA. Electronic address: youtei3@gmail.com.
Qinghao XuDepartment of Anesthesiology, University of California, San Diego, 9500 Gilman Drive, San Diego, La Jolla, CA 92093-0818, USA. Electronic address: q1xu@ucsd.edu.
Shigeki YamaguchiDepartment of Anesthesiology, Dokkyo Medical University, School of Medicine, Kitakobayashi 880, Mibu, Tochigi 321-0293, Japan. Electronic address: shigeki@dokkyomed.ac.jp.
Tony L YakshDepartment of Anesthesiology, University of California, San Diego, 9500 Gilman Drive, San Diego, La Jolla, CA 92093-0818, USA. Electronic address: tyaksh@ucsd.edu.
University of California, San Diego · USDokkyo Medical University · JP

Funding

The Pharmacology of Spinal AnalgesicsR01DA002110 · NIDA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI YAKSH, TONY L. · 1985 to 2014
$3.8M
NIDA NIH HHS DA02110NIDA NIH HHS R01 DA002110
6 · The paper itself

Abstract

Intrathecal (IT) studies have shown that several voltage sensitive calcium channels (VSCCs), such as the L-, N- and T-type may play roles in nociception and that of these only the N-type regulates primary afferent substance P (SP) release. However, the actions of other VSCCs at the spinal level are not well known. We investigated the roles of spinal P/Q- and R-type VSCCs, by IT administration of R-type (SNX-482) and P/Q-type (ω-agatoxin IVA) VSCC blockers on intraplantar formalin-evoked flinching, SP release from primary afferents and c-Fos expression in spinal dorsal horn. Intraplantar injection of formalin (2.5%, 50 μL) produced an intense, characteristic biphasic paw flinching response. In rats with IT catheters, IT SNX-482 (0.5 μg) reduced formalin-evoked paw flinching in both phase 1 and 2 compared with vehicle. Intraplantar formalin caused robust neurokinin 1 receptor (NK1r) internalization (indicating SP release) and c-Fos expression in the ipsilateral dorsal horn, which were blocked by IT SNX-482. IT ω-agatoxin IVA (0.03, 0.125 and 0.5 μg) did not reduce formalin-evoked paw flinching or c-Fos expression at any doses, with higher doses resulting in motor dysfunction. Thus, we demonstrated that blockade of spinal R-type, but not P/Q type VSCCs attenuated formalin-induced pain behavior, NK1r internalization and c-Fos expression in the superficial dorsal horn. This study supports a role for Cav2.3 in presynaptic neurotransmitter release from peptidergic nociceptive afferents and pain behaviors.

Indexed as

AnimalsCalcium Channel BlockersDose-Response Relationship, DrugInjections, SpinalMaleMovement Disordersomega-Agatoxin IVAPain MeasurementPhosphopyruvate HydrataseProtein TransportProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyReceptors, Neurokinin-1Spider VenomsSpinal CordCalcium Channel Blockersomega-Agatoxin IVAPhosphopyruvate HydrataseProto-Oncogene Proteins c-fosReceptors, Neurokinin-1SNX 482Spider VenomsSubstance Pcalcitonin gene-related peptidec-FosCGRPdorsal root gangliaDRGGPCRG protein-coupled receptorIB4immunoreactiveintrathecalIRisolectin B4ITneurokinin 1 receptorNeurokinin 1 receptorNK1rNMDAN-methyl-d-aspartateSNX-482SPSpinal cordsubstance Ptransient receptor potential protein vanilloid 1trkAtropomyosin receptor kinase ATRPV1voltage-sensitive calcium channelsVoltage sensitive calcium channelsVSCCsω-Agatoxin IVA

Identifiers

PMID23810829
PMCPMC3865027
OpenAlexW2094754857

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.