ArticleCytotechnology2014
Construction of a recombinant eukaryotic expression vector containing a leptin gene and its expression in HPMSCs.
Article in Cytotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 2 citations in OpenAlex.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptin gene fragments were amplified from human adipose tissue using reverse transcription polymerase chain reaction technology. The leptin gene was reconstructed in pIRES2-EGFP and transfected into human placenta-derived mesenchymal stem cells (HPMSCs) using a liposome-mediated method. Leptin mRNA and protein was detected in the transfected cells using RT-PCR and Western blot analysis, and the results showed that HPMSCs transfected with pIRES2-EGFP-leptin expressed significantly more leptin mRNA and protein than HPMSCs transfected with pIRES2-EGFP. EGFP expression was observed under a fluorescence microscope, and results showed the report gene to have been successfully transferred into the target cells. The biological activity of leptin and the cell proliferation activity of HPMSCs transfected with pIRES2-EGFP-leptin was detected using an MTT assay, which showed that leptin can promote the proliferation of HPMSCs. However, leptin in HPMSCs transfected with pIRES2-EGFP-leptin showed significantly more activity than HPMSCs transfected with pIRES2-EGFP. Identification of multipotency showed that HPMSCs transfected with pIRES2-EGFP-leptin maintained their multipotency.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.