ArticleNature protocols2013
Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
127 citing papers in PubMed, 274 citations in OpenAlex.
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- Statistical nonParametric Mapping Enables Rigorous Comparison of Collagen Fibril Diameter Distributions.Annals of biomedical engineering · 2026Article
- Integrating serial block-face SEM with voxel-based finite element analysis for high-fidelity micromechanical modelling of anisotropic soft tissues: application to human dermis.Biomechanics and modeling in mechanobiology · 2026Article
- 4E-BP1 acts as a molecular rheostat balancing regenerative healing and fibrotic scarring.Experimental & molecular medicine · 2026Article
- Collagen-Based Microspheres for Biomedical Applications in Drug Delivery and Tissue Engineering.Biomimetics (Basel, Switzerland) · 2026Review
- Label-free differentiation of classical and hypermobile Ehlers-Danlos syndromes using Mueller matrix polarimetry.Biophotonics discovery · 2026Article
- Grain Boundary Segregation Suppresses Local Short-Range Ordering in Nanocrystalline High-Entropy Alloys.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Atomic Force Microscopy Investigations of Collagen: From Single Molecules to Fibrils.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Regenerative Nano-scaffolds for Tissue Engineering: From Bench to Clinical Translation.Biomaterials research · 2026Article
- New Approaches Based on Serial-Block Face Electron Microscopy to Investigate the Peripheral Nervous System.Journal of the peripheral nervous system : JPNS · 2025Article
- Scleral ultrastructure in young rhesus monkeys: A stereological approach.Vision research · 2025Article
- New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation.Scientific reports · 2025Article
- Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging.Scientific reports · 2025Article
- Neural network auto-segmentation of serial-block-face scanning electron microscopy images exhibit collagen fibril structural differences with tendon type and health.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Collagen fibril formation at the plasma membrane occurs independently from collagen secretion.Wellcome open research · 2025Article
- Structural determinants of tendon multiscale mechanics and their sensitivity to mechanical stimulation during development in an embryonic chick model.Acta biomaterialia · 2024Article
- Disruption of day-to-night changes in circadian gene expression with chronic tendinopathy.The Journal of physiology · 2024Article
- Enhancing uterine receptivity for embryo implantation through controlled collagenase intervention.Life science alliance · 2024Article
- Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Collagen fibrils are the major tensile element in vertebrate tissues, in which they occur as ordered bundles in the extracellular matrix. Abnormal fibril assembly and organization results in scarring, fibrosis, poor wound healing and connective tissue diseases. Transmission electron microscopy (TEM) is used to assess the formation of the fibrils, predominantly by measuring fibril diameter. Here we describe a protocol for measuring fibril diameter as well as fibril volume fraction, mean fibril length, fibril cross-sectional shape and fibril 3D organization, all of which are major determinants of tissue function. Serial-section TEM (ssTEM) has been used to visualize fibril 3D organization in vivo. However, serial block face-scanning electron microscopy (SBF-SEM) has emerged as a time-efficient alternative to ssTEM. The protocol described below is suitable for preparing tissues for TEM and SBF-SEM (by 3View). We describe how to use 3View for studying collagen fibril organization in vivo and show how to find and track individual fibrils. The overall time scale is ~8 d from isolating the tissue to having a 3D image stack.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.