ArticleBiomedical microdevices2013
Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system.
Article in Biomedical microdevices, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- 3D Bioprinting and Microfluidic-based Devices for Cancer Detection and Drug Treatment: Focus on Prostate Cancer.Current medicinal chemistry · 2026Review
- On-chip modeling of tumor evolution: Advances, challenges and opportunities.Materials today. Bio · 2023Review
- Protein Dielectrophoresis with Gradient Array of Conductive Electrodes Sheds New Light on Empirical Theory.Analytical chemistry · 2023Article
- Real-Time Detection of Tumor Cells during Capture on a Filter Element Significantly Enhancing Detection Rate.Biosensors · 2021Article
- Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-Dielectrophoresis.Micromachines · 2020Article
- Development and Clinical Prospects of Techniques to Separate Circulating Tumor Cells from Peripheral Blood.Cancer management and research · 2020Review
- Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement.Sensors (Basel, Switzerland) · 2019Article
- Approach to classify, separate, and enrich objects in groups using ensemble sorting.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Label-free ferrohydrodynamic cell separation of circulating tumor cells.Lab on a chip · 2017Article
- Dielectrophoresis for Biomedical Sciences Applications: A Review.Sensors (Basel, Switzerland) · 2017Review
- Automated electrorotation shows electrokinetic separation of pancreatic cancer cells is robust to acquired chemotherapy resistance, serum starvation, and EMT.Biomicrofluidics · 2016Article
- Emerging microfluidic devices for cancer cells/biomarkers manipulation and detection.IET nanobiotechnology · 2016Review
- A hybrid dielectrophoretic system for trapping of microorganisms from water.Biomicrofluidics · 2015Article
- Benchtop technologies for circulating tumor cells separation based on biophysical properties.BioMed research international · 2015Review
- Enhancing sensitivity and specificity in rare cell capture microdevices with dielectrophoresis.Biomicrofluidics · 2015Article
- Dielectrophoresis for bioparticle manipulation.International journal of molecular sciences · 2014Review
- Circulating tumor cells in prostate cancer diagnosis and monitoring: an appraisal of clinical potential.Molecular diagnosis & therapy · 2014Article
- Characterization of microfluidic shear-dependent epithelial cell adhesion molecule immunocapture and enrichment of pancreatic cancer cells from blood cells with dielectrophoresis.Biomicrofluidics · 2014Article
- Circulating tumour cells-monitoring treatment response in prostate cancer.Nature reviews. Clinical oncology · 2014Review
- Article
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Authors and funding
4 authors.
Funding
Abstract
The isolation of circulating tumor cells (CTCs) from cancer patient blood is a technical challenge that is often addressed by microfluidic approaches. Two of the most prominent techniques for rare cancer cell separation, immunocapture and dielectrophoresis (DEP), are currently limited by a performance tradeoff between high efficiency and high purity. The development of a platform capable of these two performance criteria can potentially be facilitated by incorporating both DEP and immunocapture. We present a hybrid DEP-immunocapture system to characterize how DEP controls the shear-dependent capture of a prostate cancer cell line, LNCaP, and the nonspecific adhesion of peripheral blood mononuclear cells (PBMCs). Characterization of cell adhesion with and without DEP effects was performed in a Hele-Shaw flow cell that was functionalized with the prostate-specific monoclonal antibody, J591. In this model system designed to make nonspecific PBMC adhesion readily apparent, we demonstrate LNCaP enrichment from PBMCs by precisely tuning the applied AC electric field frequency to enhance immunocapture of LNCaPs and reduce nonspecific adhesion of PBMCs with positive and negative DEP, respectively. Our work shows that DEP and immunocapture techniques can work synergistically to improve cancer cell capture performance, and it informs the design of future hybrid DEP-immunocapture systems with improved CTC capture performance to facilitate research on cancer metastasis and drug therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.