ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2013
Improving molecular tools for global surveillance of measles virus.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?Preventive veterinary medicine · 2019Pooled it
- Multiple Measles Transmission Events Associated With a Single Traveler Arriving in the United States, May 2025.The Journal of infectious diseases · 2026Article
- Circulation of Measles Virus Genotype B3 in the Republic of the Congo Between 2023 and 2024: A Molecular Characterization Study.Health science reports · 2025Article
- Evaluation of Mosquito Blood Meals as a Tool for Wildlife Pathogen Surveillance.Pathogens (Basel, Switzerland) · 2025Article
- Occupational Transmission of Measles Despite COVID-19 Precautions.Pathogens (Basel, Switzerland) · 2025Article
- Building Quality Control for Molecular Assays in the Global Measles and Rubella Laboratory Network.Vaccines · 2024Review
- Rapid Detection of Measles Virus Using Reverse Transcriptase/Recombinase Polymerase Amplification Coupled with CRISPR/Cas12a and a Lateral Flow Detection: A Proof-of-Concept Study.Diagnostics (Basel, Switzerland) · 2024Article
- Genetic Characterization of the H Gene of MeV Strains (H1, B3, and D4) Recently Circulated in Iran for Improving the Molecular Measles Surveillance in the National Measles Lab.Iranian journal of public health · 2023Article
- Measles virus transmission patterns and public health responses during Operation Allies Welcome: a descriptive epidemiological study.The Lancet. Public health · 2023Article
- Detection of measles virus in Bulgaria from 2012 to 2018.Croatian medical journal · 2022Article
- Maintenance of Measles Elimination Status in the United States for 20 Years Despite Increasing Challenges.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- In-house protocol: spin-based viral RNA purification.AMB Express · 2022Article
- Evaluation of Whatman FTA cards for the preservation of yellow fever virus RNA for use in molecular diagnostics.PLoS neglected tropical diseases · 2022Article
- Vaccine-Associated Measles Encephalitis in Immunocompromised Child, California, USA.Emerging infectious diseases · 2022Article
- Diagnostic challenges and pockets of susceptibility identified during a measles outbreak, Luxembourg, 2019.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2021Article
- Measles Vaccine-Associated Rash Illness in China: an Emerging Issue in the Process of Measles Elimination.Journal of clinical microbiology · 2020Article
- Article
- Toward unified molecular surveillance of RSV: A proposal for genotype definition.Influenza and other respiratory viruses · 2020Article
- A new solid matrix for preservation of viral nucleic acid from clinical specimens at ambient temperature.Journal of virological methods · 2019Article
- Increase in Infant Measles Deaths During a Nationwide Measles Outbreak-Mongolia, 2015-2016.The Journal of infectious diseases · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 6 institutions in 6 countries.
Funding
Abstract
backgroundThe genetic characterization of wild-type measles viruses plays an important role in the description of viral transmission pathways and the verification of measles elimination. The 450 nucleotides that encode the carboxyl-terminus of the nucleoprotein (N-450) are routinely sequenced for genotype analysis.
objectivesThe objectives of this study were to develop improved primers and controls for RT-PCR reactions used for genotyping of measles samples and to develop a method to provide a convenient, safe, and inexpensive means to distribute measles RNA for RT-PCR assays and practice panels. STUDY
designA newly designed, genetically defined synthetic RNA and RNA isolated from cells infected with currently circulating genotypes were used to compare the sensitivity of primer pairs in RT-PCR and nested PCR. FTA® cards loaded with lysates of measles infected cells were tested for their ability to preserve viral RNA and destroy virus infectivity.
resultsA new primer pair, MeV216/MeV214, was able to amplify N-450 from viruses representing 10 currently circulating genotypes and a genotype A vaccine strain and demonstrated 100-fold increased sensitivity compared to the previously used primer set. A nested PCR assay further increased the sensitivity of detection from patient samples. A synthetic positive control RNA was developed that produced PCR products that are distinguishable by size from PCR products amplified from clinical samples. FTA® cards completely inactivated measles virus and stabilized RNA for at least six months.
conclusionsThese improved molecular tools will advance molecular characterization of circulating measles viruses globally and provide enhanced quality control measures.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.