ArticleJournal of chromatography. A2013
Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.
Article in Journal of chromatography. A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 39 citations in OpenAlex.
- Poly(acrylamide-Analytical chemistry · 2021Article
- Protein-induced conformational change in glycans decreases the resolution of glycoproteins in hydrophilic interaction liquid chromatography.Journal of separation science · 2021Article
- Electrophoresis of megaDalton proteins inside colloidal silica.Electrophoresis · 2019Article
- Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.Analytical chemistry · 2018Article
- Capillary HILIC-MS: A New Tool for Sensitive Top-Down Proteomics.Analytical chemistry · 2018Article
- In-column bonded phase polymerization for improved packing uniformity.Journal of separation science · 2017Article
- Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations.Chromatographia · 2017Review
- Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry.Analytical and bioanalytical chemistry · 2016Article
- Submicrometer particles and slip flow in liquid chromatography.Analytical chemistry · 2015Article
- Characterization of intact neo-glycoproteins by hydrophilic interaction liquid chromatography.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A chromatographic column of nonporous silica particles with a bonded phase of linear polyacrylamide chains is evaluated for hydrophilic interaction liquid chromatography (HILIC) of intact glycoproteins. The column is shown to retain glycoproteins significantly more strongly than non-glycoproteins. A particle diameter of 700nm gives two-fold higher resolution than does a 1.4μm particle diameter, and the column efficiency is found to be mostly limited by packing heterogeneity. LCMS is able to resolve the five glycoforms of ribonuclease B and give high quality mass spectra, but there is loss of resolution of the isomers of glycoforms due to the lower amount of TFA. Compared to two leading commercial HILIC columns operated at 60°C, the polyacrylamide column operated at 30°C provided at least two-fold higher resolution for intact ribonuclease B, and showed peaks for glycoforms of prostate specific antigen, although not resolved.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.