ArticleLeukemia research2013
Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.
Article in Leukemia research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.
- The diagnostic value of DNA methylation in leukemia: a systematic review and meta-analysis.PloS one · 2014Pooled it
- Article
- Hyper-radiosensitivity affects low-dose acute myeloid leukemia incidence in a mathematical model.Radiation and environmental biophysics · 2022Article
- Modeling low-dose radiation-induced acute myeloid leukemia in male CBA/H mice.Radiation and environmental biophysics · 2021Article
- Kras mutations and PU.1 promoter methylation are new pathways in murine radiation-induced AML.Carcinogenesis · 2020Article
- Murine Models of Acute Myeloid Leukaemia.International journal of molecular sciences · 2019Review
- Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis.Leukemia · 2018Article
- MASM, a Matrine Derivative, Offers Radioprotection by Modulating Lethal Total-Body Irradiation-Induced Multiple Signaling Pathways in Wistar Rats.Molecules (Basel, Switzerland) · 2016Article
- Article
- PU.1 downregulation in murine radiation-induced acute myeloid leukaemia (AML): from molecular mechanism to human AML.Carcinogenesis · 2015Review
- The Lowest Radiation Dose Having Molecular Changes in the Living Body.Dose-response : a publication of International Hormesis SocietyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CBA/H mouse model of radiation-induced acute myeloid leukaemia (rAML) has been studied for decades to bring to light the molecular mechanisms associated with multistage carcinogenesis. A specific interstitial deletion of chromosome 2 found in a high proportion of rAML is recognised as the initiating event. The deletion leads to the loss of Sfpi, a gene essential for haematopoietic development. Its product, the transcription factor PU.1 acts as a tumour suppressor in this model. Although the deletion can be detected early following ionising radiation exposure by cytogenetic techniques, precise characterisation of the haematopoietic cells carrying the deletion and the study of their fate in vivo cannot be achieved. Here, using a genetically engineered C57BL/6 mouse model expressing the GFP fluorescent molecule under the control of the Sfpi1 promoter, which we have bred onto the rAML-susceptible CBA/H strain, we demonstrate that GFP expression did not interfere with X-ray induced leukaemia incidence and that GFP fluorescence in live leukaemic cells is a surrogate marker of radiation-induced chromosome 2 deletions with or without point mutations on the remaining allele of the Sfpi1 gene. This study presents the first experimental evidence for the detection of this leukaemia initiating event in live leukemic cells.
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