ArticleBMC cancer2013
DNA methylation analysis reveals distinct methylation signatures in pediatric germ cell tumors.
Article in BMC cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Report From the International Society of Urological Pathology (ISUP) Consultation Conference on Molecular Pathology of Urogenital Cancers: IV: Current and Future Utilization of Molecular-Genetic Tests for Testicular Germ Cell Tumors.The American journal of surgical pathology · 2020Guideline
- Cripto: Expression, epigenetic regulation and potential diagnostic use in testicular germ cell tumors.Molecular oncology · 2016Trial
- Article
- Case Report: Prepubertal-type testicular teratoma with local metastasis in a postpubertal patient.Frontiers in oncology · 2025Article
- Review
- Mature and immature ovarian teratomas share methylation profiles of imprinted genes: a MS-MLPA analysis.Virchows Archiv : an international journal of pathology · 2023Article
- In Utero Programming of Testicular Cancer.Journal of developmental biology · 2021Review
- Molecular Pathology and Targeted Therapies for Personalized Management of Central Nervous System Germinoma.Journal of personalized medicine · 2021Review
- Survival differences by race/ethnicity among children and adolescents diagnosed with germ cell tumors.International journal of cancer · 2020Article
- Predicting Gonadal Germ Cell Cancer in People with Disorders of Sex Development; Insights from Developmental Biology.International journal of molecular sciences · 2019Review
- Human Germ Cell Tumors are Developmental Cancers: Impact of Epigenetics on Pathobiology and Clinic.International journal of molecular sciences · 2019Review
- Testicular cancer.Nature reviews. Disease primers · 2018Review
- Differences in DNA methylation profiles by histologic subtype of paediatric germ cell tumours: a report from the Children's Oncology Group.British journal of cancer · 2018Article
- EGF Receptor and mTORC1 Are Novel Therapeutic Targets in Nonseminomatous Germ Cell Tumors.Molecular cancer therapeutics · 2018Article
- Article
- Variants in BAK1, SPRY4, and GAB2 are associated with pediatric germ cell tumors: A report from the children's oncology group.Genes, chromosomes & cancer · 2017Article
- Risk of malignant childhood germ cell tumors in relation to demographic, gestational, and perinatal characteristics.Cancer epidemiology · 2017Article
- Imprints and DPPA3 are bypassed during pluripotency- and differentiation-coupled methylation reprogramming in testicular germ cell tumors.Genome research · 2016Article
- Parent-of-origin effects of A1CF and AGO2 on testicular germ-cell tumors, testicular abnormalities, and fertilization bias.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Distinct subcellular localization and potential role of LINE1-ORF1P in meiotic oocytes.Histochemistry and cell biology · 2016Article
Corrections and comments
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Authors and funding
11 authors at 8 institutions in 1 country.
Funding
Abstract
backgroundAberrant DNA methylation is a prominent feature of many cancers, and may be especially relevant in germ cell tumors (GCTs) due to the extensive epigenetic reprogramming that occurs in the germ line during normal development.
methodsWe used the Illumina GoldenGate Cancer Methylation Panel to compare DNA methylation in the three main histologic subtypes of pediatric GCTs (germinoma, teratoma and yolk sac tumor (YST); N = 51) and used recursively partitioned mixture models (RPMM) to test associations between methylation pattern and tumor and demographic characteristics. We identified genes and pathways that were differentially methylated using generalized linear models and Ingenuity Pathway Analysis. We also measured global DNA methylation at LINE1 elements and evaluated methylation at selected imprinted loci using pyrosequencing.
resultsMethylation patterns differed by tumor histology, with 18/19 YSTs forming a distinct methylation class. Four pathways showed significant enrichment for YSTs, including a human embryonic stem cell pluripotency pathway. We identified 190 CpG loci with significant methylation differences in mature and immature teratomas (q < 0.05), including a number of CpGs in stem cell and pluripotency-related pathways. Both YST and germinoma showed significantly lower methylation at LINE1 elements compared with normal adjacent tissue while there was no difference between teratoma (mature and immature) and normal tissue. DNA methylation at imprinted loci differed significantly by tumor histology and location.
conclusionUnderstanding methylation patterns may identify the developmental stage at which the GCT arose and the at-risk period when environmental exposures could be most harmful. Further, identification of relevant genetic pathways could lead to the development of new targets for therapy.
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