ArticleMicrobial cell factories2013
Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Harnessing the thermotolerant methylotroph Bacillus methanolicus for methanol-based synthetic L-proline production.Microbial cell factories · 2026Article
- Characterization of bacterial endophytes isolated fromFrontiers in microbiomes · 2026Article
- Rapid screening of point mutations by mismatch amplification mutation assay PCR.Applied microbiology and biotechnology · 2024Article
- Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds byFrontiers in microbiology · 2022Review
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Improved Plasmid-Based Inducible and Constitutive Gene Expression inMicroorganisms · 2021Article
- Exploring the Potential ofFrontiers in bioengineering and biotechnology · 2021Article
- Improvement of l-arginine production by in silico genome-scale metabolic network model guided genetic engineering.3 Biotech · 2020Article
- Function of L-Pipecolic Acid as Compatible Solute inFrontiers in microbiology · 2019Article
- One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst.Scientific reports · 2018Article
- CombinedStructure (London, England : 1993) · 2018Article
- Fermentative Production ofFrontiers in bioengineering and biotechnology · 2018Article
- Physiological Response ofFrontiers in microbiology · 2018Article
- Recombinant Protein Expression System inFrontiers in microbiology · 2018Review
- Redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2017Article
- Functional Characterization of Four Putative δFrontiers in microbiology · 2017Article
- A new genome-scale metabolic model ofBiotechnology for biofuels · 2017Article
- Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.Applied and environmental microbiology · 2016Article
- Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum.BMC microbiology · 2016Article
- Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe soil bacterium Corynebacterium glutamicum, best known for its glutamate producing ability, is suitable as a producer of a variety of bioproducts. Glutamate is the precursor of the amino acid proline. Proline biosynthesis typically involves three enzymes and a spontaneous cyclisation reaction. Alternatively, proline can be synthesised from ornithine, an intermediate of arginine biosynthesis. The direct conversion of ornithine to proline is catalysed by ornithine cyclodeaminase. An ornithine overproducing platform strain with deletions of argR and argF (ORN1) has been employed for production of derived compounds such as putrescine. By heterologous expression of ocd this platform strain can be engineered further for proline production.
resultsPlasmid-based expression of ocd encoding the putative ornithine cyclodeaminase of C. glutamicum did not result in detectable proline accumulation in the culture medium. However, plasmid-based expression of ocd from Pseudomonas putida resulted in proline production with yields up to 0.31 ± 0.01 g proline/g glucose. Overexpression of the gene encoding a feedback-alleviated N-acetylglutamate kinase further increased proline production to 0.36 ± 0.01 g/g. In addition, feedback-alleviation of N-acetylglutamate kinase entailed growth-coupled production of proline and reduced the accumulation of by-products in the culture medium.
conclusionsThe product spectrum of the platform strain C. glutamicum ORN1 was expanded to include the amino acid L-proline. Upon further development of the ornithine overproducing platform strain, industrial production of amino acids of the glutamate family and derived bioproducts such as diamines might become within reach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.