ArticlePloS one2013
Purification and Characterization of Biofilm-Associated EPS Exopolysaccharides from ESKAPE Organisms and Other Pathogens.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 224 citations in OpenAlex.
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- Characterization of biofilm and exopolysaccharide production in Bacillus strains isolated from the bovine uterus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Antibacterial and antibiofilm activity of nanoparticles inRSC advances · 2025Article
- Comparative Analysis of Biofilm Formation and Antibiotic Resistance in Five ESKAPE Pathogen Species from a Tertiary Hospital in Bangladesh.Antibiotics (Basel, Switzerland) · 2025Article
- Isolation of Aerobic Heterotrophic Bacteria from a Microbial Mat with the Ability to Grow on and Remove Hexavalent Chromium through Biosorption and Bioreduction.Applied biochemistry and biotechnology · 2025Article
- Removal of hexavalent chromium by a microbial mat from a mining site under anaerobic conditions.Frontiers in bioengineering and biotechnology · 2025Article
- Advances in alginate biosynthesis: regulation and production inFrontiers in bioengineering and biotechnology · 2025Review
- Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme.NPJ biofilms and microbiomes · 2024Article
- The activity of hydrolytic enzymes and antibiotics against biofilms of bacteria isolated from industrial-scale cooling towers.Microbial cell factories · 2024Article
- Cellular Effects ofInternational journal of molecular sciences · 2024Article
- Current treatment strategies for targeting virulence factors and biofilm formation inFuture microbiology · 2024Review
- Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances.Journal of pharmaceutical analysis · 2024Review
- Drug Repurposing: Research Progress of Niclosamide and Its Derivatives on Antibacterial Activity.Infection and drug resistance · 2024Review
- Alginate-like exopolysaccharides extracted from different waste sludges exhibit varying physicochemical and material properties.Frontiers in microbiology · 2024Article
- Glycosyl hydrolase fromBiofilm · 2023Article
- Biochemical and microbiological characterization of a thermotolerant bacterial consortium involved in the corrosion of Aluminum Alloy 7075.World journal of microbiology & biotechnology · 2023Article
- Exploring the enzymatic activity of depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2.Virus research · 2023Article
- Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms.Food science and biotechnology · 2023Review
- Changes in the Ultrastructure ofMicroorganisms · 2023Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
In bacterial biofilms, high molecular weight, secreted exopolysaccharides can serve as a scaffold to which additional carbohydrates, proteins, lipids, and nucleic acids adhere, forming the matrix of the developing biofilm. Here we report methods to extract and purify high molecular weight (>15 kDa) exopolysaccharides from biofilms of eight human pathogens, including species of Staphylcococcus, Klebsiella, Acinetobacter, Pseudomonas, and a toxigenic strain of Escherichia coli O157:H7. Glycosyl composition analysis indicated a high total mannose content across all strains with P. aeruginosa and A. baumannii exopolysaccharides comprised of 80-90% mannose, K. pneumoniae and S. epidermidis strains containing 40-50% mannose, and E. coli with ∼10% mannose. Galactose and glucose were also present in all eight strains, usually as the second and third most abundant carbohydrates. N-acetyl-glucosamine and galacturonic acid were found in 6 of 8 strains, while arabinose, fucose, rhamnose, and xylose were found in 5 of 8 strains. For linkage analysis, 33 distinct residue-linkage combinations were detected with the most abundant being mannose-linked moieties, in line with the composition analysis. The exopolysaccharides of two P. aeruginosa strains analyzed were consistent with the Psl carbohydrate, but not Pel or alginate. The S. epidermidis strain had a composition rich in mannose and glucose, which is consistent with the previously described slime associated antigen (SAA) and the extracellular slime substance (ESS), respectively, but no polysaccharide intracellular adhesion (PIA) was detected. The high molecular weight exopolysaccharides from E. coli, K. pneumoniae, and A. baumannii appear to be novel, based on composition and/or ratio analysis of carbohydrates.
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