ArticlePloS one2013
Lifelong Physical Activity Prevents Aging-Associated Insulin Resistance in Human Skeletal Muscle Myotubes via Increased Glucose Transporter Expression.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men.In vitro cellular & developmental biology. Animal · 2026Article
- Age-Related Anabolic Resistance: Nutritional and Exercise Strategies, and Potential Relevance to Life-Long Exercisers.Nutrients · 2025Review
- Effects of aerobic, resistance, interval, and combined training on glucose metabolism in older adults: insights into type, dose, and mechanism.Frontiers in physiology · 2025Review
- Lean Mass Longitudinally Confounds Sedentary Time and Physical Activity With Blood Pressure Progression in 2513 Children.Journal of cachexia, sarcopenia and muscle · 2024Article
- Effects of accelerometer-based sedentary time and physical activity on DEXA-measured fat mass in 6059 children.Nature communications · 2023Article
- Nutri-stress, mitochondrial dysfunction, and insulin resistance-role of heat shock proteins.Cell stress & chaperones · 2023Review
- Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice.Nutrients · 2022Article
- Improved Aerobic Capacity and Adipokine Profile Together with Weight Loss Improve Glycemic Control without Changes in Skeletal Muscle GLUT-4 Gene Expression in Middle-Aged Subjects with Impaired Glucose Tolerance.International journal of environmental research and public health · 2022Article
- Phenotypic and genotypic changes in obesity and type 2 diabetes of male KK mice with aging.Experimental animals · 2022Article
- Metformin and Insulin Resistance: A Review of the Underlying Mechanisms behind Changes in GLUT4-Mediated Glucose Transport.International journal of molecular sciences · 2022Review
- Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats.Nutrients · 2021Article
- Pathophysiology of Physical Inactivity-Dependent Insulin Resistance: A Theoretical Mechanistic Review Emphasizing Clinical Evidence.Journal of diabetes research · 2021Review
- Substrate oxidation in primary human skeletal muscle cells is influenced by donor age.Cell and tissue research · 2020Article
- Effects of resistance exercise on adipokine factors and body composition in pre- and postmenopausal women.Journal of exercise rehabilitation · 2019Article
- Physical activity and muscle-brain crosstalk.Nature reviews. Endocrinology · 2019Review
- Aerobic and resistance exercise training reverses age-dependent decline in NADPhysiological reports · 2019Article
- Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training.International journal of molecular sciences · 2019Article
- Physical activity and sedentary behaviour research in Thailand: a systematic scoping review.BMC public health · 2018Article
- Age-related impairment of pancreatic Beta-cell function: pathophysiological and cellular mechanisms.Frontiers in endocrinology · 2014Review
- Effects of Electrical Stimulation on Skeletal Muscle of Old Sedentary People.Gerontology & geriatric medicineArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both aging and physical inactivity are associated with increased development of insulin resistance whereas physical activity has been shown to promote increased insulin sensitivity. Here we investigated the effects of physical activity level on aging-associated insulin resistance in myotubes derived from human skeletal muscle satellite cells. Satellite cells were obtained from young (22 yrs) normally active or middle-aged (56.6 yrs) individuals who were either lifelong sedentary or lifelong active. Both middle-aged sedentary and middle-aged active myotubes had increased p21 and myosin heavy chain protein expression. Interestingly MHCIIa was increased only in myotubes from middle-aged active individuals. Middle-aged sedentary cells had intact insulin-stimulated Akt phosphorylation however, the same cell showed ablated insulin-stimulated glucose uptake and GLUT4 translocation to the plasma membrane. On the other hand, middle-aged active cells retained both insulin-stimulated increases in glucose uptake and GLUT4 translocation to the plasma membrane. Middle-aged active cells also had significantly higher mRNA expression of GLUT1 and GLUT4 compared to middle-aged sedentary cells, and significantly higher GLUT4 protein. It is likely that physical activity induces a number of stable adaptations, including increased GLUT4 expression that are retained in cells ex vivo and protect, or delay the onset of middle-aged-associated insulin resistance. Additionally, a sedentary lifestyle has an impact on the metabolism of human myotubes during aging and may contribute to aging-associated insulin resistance through impaired GLUT4 localization.
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