ArticlePloS one2013
Optimization of Multiple Pathogen Detection Using the TaqMan Array Card: Application for a Population-Based Study of Neonatal Infection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 100 citations in OpenAlex.
- An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.BMC infectious diseases · 2021Trial
- Prevalence of Group AThe American journal of tropical medicine and hygiene · 2020Trial
- Performance evaluation of TaqMan Array Card real-time PCR for multi-pathogen detection in acute undifferentiated febrile illness.BMC infectious diseases · 2026Article
- Whole-genome sequencing-based pathogen characterization for streptococcal infection directly from positive blood culture samples.Journal of clinical microbiology · 2026Article
- No Histopathological Evidence of Inflammation Despite Molecular Detection ofPathogens (Basel, Switzerland) · 2025Article
- Comparative evaluation of Biofire Joint Infection Panel, Sepsitest 16S/18S rDNA PCR, and culture to identify microorganisms in explanted heart valves.Microbiology spectrum · 2025Article
- First Report ofMicroorganisms · 2025Article
- Prospective evaluation of real-world performance and clinical impact of the Biofire FilmArray joint infection panel.Microbiology spectrum · 2025Article
- Respiratory pathogens detected in children aged <5 years hospitalized with severe respiratory illness, South Africa, 2017.Frontiers in pediatrics · 2025Article
- Comparison of qPCR and chromogenic culture methods for rapid detection of group B streptococcus colonization in Vietnamese pregnant women.Practical laboratory medicine · 2024Article
- Stability characterizations of feed-based bivalent vaccine containing inactivated Streptococcus agalactiae and Aeromonas hydrophila against streptococcosis and Aeromonas infections in red hybrid tilapia (Oreochromis sp.).Archives of microbiology · 2024Article
- Evaluation of a multiplex-qPCR for paediatric pleural empyema-An observational study in hospitalised children.PloS one · 2024Observational
- Multiplexed bacterial pathogen detection and clinical characteristics of orthopedic infection in hospitalized patients.Frontiers in cellular and infection microbiology · 2024Article
- Simultaneous detection and quantification of multiple pathogen targets in wastewater.medRxiv : the preprint server for health sciences · 2023Article
- Article
- Performance and Hypothetical Impact on Joint Infection Management of the BioFire Joint Infection Panel: a Retrospective Analysis.Journal of clinical microbiology · 2023Article
- Do NAAT-Based Methods Increase the Diagnostic Sensitivity ofDiagnostics (Basel, Switzerland) · 2023Article
- Pathogens Identified by Minimally Invasive Tissue Sampling in India and Pakistan From Preterm Neonatal Deaths: The PURPOSE Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Observational
- The causes of preterm neonatal deaths in India and Pakistan (PURPOSe): a prospective cohort study.The Lancet. Global health · 2022Article
- The causes of stillbirths in south Asia: results from a prospective study in India and Pakistan (PURPOSe).The Lancet. Global health · 2022Article
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of etiology remains a significant challenge in the diagnosis of infectious diseases, particularly in resource-poor settings. Viral, bacterial, and fungal pathogens, as well as parasites, play a role for many syndromes, and optimizing a single diagnostic system to detect a range of pathogens is challenging. The TaqMan Array Card (TAC) is a multiple-pathogen detection method that has previously been identified as a valuable technique for determining etiology of infections and holds promise for expanded use in clinical microbiology laboratories and surveillance studies. We selected TAC for use in the Aetiology of Neonatal Infection in South Asia (ANISA) study for identifying etiologies of severe disease in neonates in Bangladesh, India, and Pakistan. Here we report optimization of TAC to improve pathogen detection and overcome technical challenges associated with use of this technology in a large-scale surveillance study. Specifically, we increased the number of assay replicates, implemented a more robust RT-qPCR enzyme formulation, and adopted a more efficient method for extraction of total nucleic acid from blood specimens. We also report the development and analytical validation of ten new assays for use in the ANISA study. Based on these data, we revised the study-specific TACs for detection of 22 pathogens in NP/OP swabs and 12 pathogens in blood specimens as well as two control reactions (internal positive control and human nucleic acid control) for each specimen type. The cumulative improvements realized through these optimization studies will benefit ANISA and perhaps other studies utilizing multiple-pathogen detection approaches. These lessons may also contribute to the expansion of TAC technology to the clinical setting.
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