ArticleFrontiers in immunology2013
An improved strategy to recover large fragments of functional human neutrophil extracellular traps.
Article in Frontiers in immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- In vitro induction of NETosis: Comprehensive live imaging comparison and systematic review.PloS one · 2017Pooled it
- p1/s1, a 3'-nucleotidase/nuclease, allows Leishmania major to circumvent host innate immune response mechanisms.PLoS pathogens · 2026Article
- Neutrophil extracellular traps promote neuronal ferroptosis through STING-mediated AMPK dysregulation after traumatic brain injury.International journal of biological sciences · 2026Article
- Article
- Neutrophil extracellular traps have active DNAzymes that promote bactericidal activity.Nucleic acids research · 2025Article
- Equine neutrophils selectively release neutrophil extracellular traps in response to chemical and bacterial agonists.Frontiers in veterinary science · 2025Article
- IL-4R and CXCR2 Contribute to Downregulating Neutrophil-Mediated Response in the Early Stage of Fungal Extract-Induced Allergic Airway Inflammation.Biomedicines · 2024Article
- Cyclic stretch enhances neutrophil extracellular trap formation.BMC biology · 2024Article
- Neutrophil Extracellular Traps have DNAzyme activity that drives bactericidal potential.bioRxiv : the preprint server for biology · 2023Article
- Short-Term Effect of SARS-CoV-2 Spike Protein Receptor-Binding Domain-Specific Antibody Induction on Neutrophil-Mediated Immune Response in Mice.International journal of molecular sciences · 2022Article
- Serine protease inhibitor derived fromFrontiers in cellular and infection microbiology · 2022Article
- Neutrophil extracellular traps enhance macrophage killing of bacterial pathogens.Science advances · 2021Article
- Neutrophil extracellular traps impair intestinal barrier functions in sepsis by regulating TLR9-mediated endoplasmic reticulum stress pathway.Cell death & disease · 2021Article
- In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Antimicrobial Activity of Neutrophils Against Mycobacteria.Frontiers in immunology · 2021Review
- Neutrophil extracellular traps modulate inflammatory markers and uptake of oxidized LDL by human and murine macrophages.PloS one · 2021Article
- Neutrophil Myeloperoxidase Derived Chlorolipid Production During Bacteria Exposure.Frontiers in immunology · 2021Article
- Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils.Frontiers in immunology · 2021Article
- Neutralizing the pathological effects of extracellular histones with small polyanions.Nature communications · 2020Article
- A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries.Experimental cell research · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Netosis is a recently described neutrophil function that leads to the release of neutrophil extracellular traps (NETs) in response to various stimuli. NETs are filaments of decondensed chromatin associated with granular proteins. In addition to their role against microorganisms, NETs have been implicated in autoimmunity, thrombosis, and tissue injury. Access to a standardized source of isolated NETs is needed to better analyze the roles of NETs. The aim of this study was to develop a procedure yielding soluble, well-characterized NET preparations from fresh human neutrophils. The calcium ionophore A23187 was chosen to induce netosis, and the restriction enzyme AluI was used to prepare large NET fragments. DNA and proteins were detected by electrophoresis and specific labeling. Some NET proteins [histone 3, lactoferrin (LF)] were quantified by western blotting, and double-stranded DNA (dsDNA) was quantified by immunofluorescence. Co-existence of dsDNA and neutrophil proteins confirmed the quality of the NET preparations. Their biological activity was checked by measuring elastase (ELA) activity and bacterial killing against various strains. Interindividual differences in histone 3, LF, ELA, and dsDNA relative contents were observed in isolated NETs. However, the reproducibility of NET preparation and characterization was validated, suggesting that this interindividual variability was rather related to donor variation than to technical bias. This standardized protocol is suitable for producing, isolating, and quantifying functional NETs that could serve as a tool for studying NET effects on immune cells and tissues.
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