ArticleObesity (Silver Spring, Md.)2014
CRF type 2 receptors mediate the metabolic effects of ghrelin in C2C12 cells.
Article in Obesity (Silver Spring, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Variations of Corticotropin-Releasing Factor Receptor 1α Contribute to the Blunted HPA Axis Responses to Hypoxia in Plateau Mammals.Neuroscience bulletin · 2026Article
- Exercise is required to maintain unacylated ghrelin response in adult male rat skeletal muscle, regardless of dietary fat consumption.The Journal of pharmacology and experimental therapeutics · 2025Article
- Regulation of peripheral tissue substrate metabolism by the gut-derived hormone ghrelin.Metabolism open · 2024Review
- The effects of diet and chronic exercise on skeletal muscle ghrelin response.Metabolism open · 2022Article
- Regulation of adipose tissue lipolysis by ghrelin is impaired with high-fat diet feeding and is not restored with exercise.Adipocyte · 2021Article
- Ghrelin, via corticotropin-releasing factor receptors, reduces glucose uptake and increases lipid content in mouse myoblasts cells.Physiological reports · 2021Article
- Protective Effects of Ghrelin on Fasting-Induced Muscle Atrophy in Aging Mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2020Article
- Unacylated ghrelin stimulates fatty acid oxidation to protect skeletal muscle against palmitate-induced impairment of insulin action in lean but not high-fat fed rats.Metabolism open · 2020Article
- Ghrelin forms in the modulation of energy balance and metabolism.Eating and weight disorders : EWD · 2019Review
- Ghrelin stimulates fatty acid oxidation and inhibits lipolysis in isolated muscle from male rats.Physiological reports · 2019Article
- Acylated and unacylated ghrelin do not directly stimulate glucose transport in isolated rodent skeletal muscle.Physiological reports · 2017Article
- Ghrelin prevents tumour- and cisplatin-induced muscle wasting: characterization of multiple mechanisms involved.Journal of cachexia, sarcopenia and muscle · 2015Article
- Taking two to tango: a role for ghrelin receptor heterodimerization in stress and reward.Frontiers in neuroscience · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
objectiveGhrelin is known to regulate appetite control and cellular metabolism. The corticotropin-releasing factor (CRF) family is also known to regulate energy balance. In this study, the links between ghrelin and the CRF family in C2C12 cells, a mouse myoblast cell line was investigated. DESIGN AND
methodsC2C12 cells were treated with ghrelin in the presence or absence of CRF receptor antagonists and then subjected to different metabolic analyses.
resultsGhrelin enhanced glucose uptake by C2C12 cells, induced GLUT4 translocation to the cell surface and decreased RBP4 expression. A CRF-R2 selective antagonist, anti-sauvagine-30, blocked ghrelin-induced glucose uptake, Ghrelin upregulated CRF-R2 but not CRF-R1 levels. Moreover, ghrelin-treated C2C12 cells displayed a cAMP and pERK activation in response to Ucn3, a CRF-R2 specific ligand, but not in response to CRF or stressin, CRF-R1 specific ligands. Ghrelin also induced UCP2 and UCP3 expression, which were blocked by anti- sauvagine-30. Ghrelin did not induce fatty acids uptake by C2C12 cells or ACC expression. Even though C2C12 cells clearly exhibited responses to ghrelin, the known ghrelin receptor, GHSR1a, was not detectable in C2C12 cells.
conclusionThe results suggest that, ghrelin plays a role in regulating muscle glucose and, raise the possibility that suppression of the CRF-R2 pathway might provide benefits in high ghrelin states.
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