ArticleThe Journal of biological chemistry2013
Cytotoxic necrotizing factor-Y boosts Yersinia effector translocation by activating Rac protein.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Live imaging of Yersinia translocon formation and immune recognition in host cells.PLoS pathogens · 2022Article
- Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica.Science China. Life sciences · 2022Article
- The Cytotoxic Necrotizing Factors (CNFs)-A Family of Rho GTPase-Activating Bacterial Exotoxins.Toxins · 2021Review
- Crystal structure of bacterial cytotoxic necrotizing factor CNFThe EMBO journal · 2021Article
- Far Eastern Scarlet-Like Fever is a Special Clinical and Epidemic Manifestation ofPathogens (Basel, Switzerland) · 2020Review
- An RNA thermometer dictates production of a secreted bacterial toxin.PLoS pathogens · 2020Article
- Article
- Visualization of translocons in Yersinia type III protein secretion machines during host cell infection.PLoS pathogens · 2018Article
- Loss of CNFY toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency.PLoS pathogens · 2018Article
- Immunomodulatory Yersinia outer proteins (Yops)-useful tools for bacteria and humans alike.Virulence · 2017Review
- Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Analysis of differentially expressed proteins in Yersinia enterocolitica-infected HeLa cells.Biochimica et biophysica acta · 2016Article
- Yersinia versus host immunity: how a pathogen evades or triggers a protective response.Current opinion in microbiology · 2016Review
- Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions.Journal of visualized experiments : JoVE · 2015Article
- Common Virulence Factors and Tissue Targets of Entomopathogenic Bacteria for Biological Control of Lepidopteran Pests.Insects · 2014Article
- The cytotoxic necrotizing factor of Yersinia pseudotuberculosis (CNFY) enhances inflammation and Yop delivery during infection by activation of Rho GTPases.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenic Yersinia spp. translocate the effectors YopT, YopE, and YopO/YpkA into target cells to inactivate Rho family GTP-binding proteins and block immune responses. Some Yersinia spp. also secrete the Rho protein activator cytotoxic necrotizing factor-Y (CNF-Y), but it has been unclear how the bacteria may benefit from Rho protein activation. We show here that CNF-Y increases Yop translocation in Yersinia enterocolitica-infected cells up to 5-fold. CNF-Y strongly activated RhoA and also delayed in time Rac1 and Cdc42, but when individually expressed, constitutively active mutants of Rac1, but not of RhoA, increased Yop translocation. Consistently, knock-out or knockdown of Rac1 but not of RhoA, -B, or -C inhibited Yersinia effector translocation in CNF-Y-treated and control cells. Activation or knockdown of Cdc42 also affected Yop translocation but much less efficiently than Rac. The increase in Yop translocation induced by CNF-Y was essentially independent of the presence of YopE, YopT, or YopO in the infecting Yersinia strain, indicating that none of the Yops reported to inhibit translocation could reverse the CNF-Y effect. In summary, the CNF-Y activity of Yersinia strongly enhances Yop translocation through activation of Rac.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.