ArticlePlant physiology2013
A rice virescent-yellow leaf mutant reveals new insights into the role and assembly of plastid caseinolytic protease in higher plants.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.
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64 citing papers in PubMed, 1 synthesis or guideline pooled it, 136 citations in OpenAlex.
- A Systematic View Exploring the Role of Chloroplasts in Plant Abiotic Stress Responses.BioMed research international · 2019Pooled it
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- Phytotoxic effects of cigarette smoke on indoor plantFrontiers in plant science · 2025Article
- Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant inInternational journal of molecular sciences · 2024Article
- Map-based cloning revealed BhAPRR2 gene regulating the black peel formation of mature fruit in wax gourd (Benincasa hispida).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- Integrating Physiology, Cytology, and Transcriptome to Reveal the Leaf Variegation Mechanism inBiomolecules · 2024Article
- Molecular Mechanisms of Chlorophyll Deficiency inPlants (Basel, Switzerland) · 2024Article
- Silencing an ATP-Dependent Caseinolytic Protease Proteolytic Subunit Gene Enhances the Resistance of Rice toInternational journal of molecular sciences · 2024Article
- AmCBF1 activates the expression of GhClpR1 to mediate dark-green leaves in cotton (Gossypium hirsutum).Plant cell reports · 2024Article
- Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice.Plants (Basel, Switzerland) · 2023Article
- A large deletion conferring pale green leaves of maize.BMC plant biology · 2023Article
- Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (Plants (Basel, Switzerland) · 2023Article
- Genetic mapping and molecular characterization of the delayed green geneFrontiers in plant science · 2023Article
- Identification of quantitative trait loci for important agronomic traits using chromosome segment substitution lines from aMolecular breeding : new strategies in plant improvement · 2022Article
- Cia Zeaxanthin Biosynthesis,International journal of molecular sciences · 2022Article
- Integrating physiology, genetics, and transcriptome to decipher a new thermo-sensitive and light-sensitive virescent leaf gene mutant in cucumber.Frontiers in plant science · 2022Article
- Mapping and functional verification of leaf yellowing genes in watermelon during whole growth period.Frontiers in plant science · 2022Article
- Identification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
4 more citing papers are in PubMed but not listed here.
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17 authors at 2 institutions in 1 country.
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Abstract
The plastidic caseinolytic protease (Clp) of higher plants is an evolutionarily conserved protein degradation apparatus composed of a proteolytic core complex (the P and R rings) and a set of accessory proteins (ClpT, ClpC, and ClpS). The role and molecular composition of Clps in higher plants has just begun to be unraveled, mostly from studies with the model dicotyledonous plant Arabidopsis (Arabidopsis thaliana). In this work, we isolated a virescent yellow leaf (vyl) mutant in rice (Oryza sativa), which produces chlorotic leaves throughout the entire growth period. The young chlorotic leaves turn green in later developmental stages, accompanied by alterations in chlorophyll accumulation, chloroplast ultrastructure, and the expression of chloroplast development- and photosynthesis-related genes. Positional cloning revealed that the VYL gene encodes a protein homologous to the Arabidopsis ClpP6 subunit and that it is targeted to the chloroplast. VYL expression is constitutive in most tissues examined but most abundant in leaf sections containing chloroplasts in early stages of development. The mutation in vyl causes premature termination of the predicted gene product and loss of the conserved catalytic triad (serine-histidine-aspartate) and the polypeptide-binding site of VYL. Using a tandem affinity purification approach and mass spectrometry analysis, we identified OsClpP4 as a VYL-associated protein in vivo. In addition, yeast two-hybrid assays demonstrated that VYL directly interacts with OsClpP3 and OsClpP4. Furthermore, we found that OsClpP3 directly interacts with OsClpT, that OsClpP4 directly interacts with OsClpP5 and OsClpT, and that both OsClpP4 and OsClpT can homodimerize. Together, our data provide new insights into the function, assembly, and regulation of Clps in higher plants.
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