ArticleVirology journal2013
Transcriptional profiling of Drosophila S2 cells in early response to Drosophila C virus.
Article in Virology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
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- Viral susceptibility across host species is largely independent of dietary protein to carbohydrate ratios.Journal of evolutionary biology · 2021Article
- Hazardous effects of octopamine receptor agonists on altering metabolism-related genes and behavior of Drosophila melanogaster.Chemosphere · 2020Article
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- Antimicrobial Peptides as Potential Antiviral Factors in Insect Antiviral Immune Response.Frontiers in immunology · 2020Review
- Endogenous non-retroviral elements in genomes of Aedes mosquitoes and vector competence.Emerging microbes & infections · 2019Review
- Evolution of longevity improves immunity inEvolution letters · 2018Article
- Isolation of a natural DNA virus of Drosophila melanogaster, and characterisation of host resistance and immune responses.PLoS pathogens · 2018Article
- Complex Coding and Regulatory Polymorphisms in a Restriction Factor Determine the Susceptibility ofGenetics · 2017Article
- Disruption of Stress Granule Formation by the Multifunctional Cricket Paralysis Virus 1A Protein.Journal of virology · 2017Article
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- A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila.PLoS pathogens · 2016Article
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- SUMO-Enriched Proteome for Drosophila Innate Immune Response.G3 (Bethesda, Md.) · 2015Article
- Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference.Scientific reports · 2015Article
- RNAi and Antiviral Defense in the Honey Bee.Journal of immunology research · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe innate immune response like phagocytosis, encapsulation and antimicrobial peptide (AMP) production often occur in the early stage of host-pathogen interactions in Drosophila melanogaster. To investigate the Drosophila early immune response to Drosophila C virus, we characterized the DCV infection-response transcriptome of Drosophila Schneider 2 (S2) cells at one hour post inoculation.
methodThe total RNA was extracted from treated S2 cells by using Trizol reagent and then analyzed by CapitalBio Corp for Drosophila GeneChip (Affymetrix) assay. Then the results of signaling pathway and protein interaction about these genes were analyzed by MAS 3.0 software.
resultsMost significantly affected genes (656 genes) by DCV infection were regulated as the same way in inactivated DCV treatment, but inactivated white spot syndrome virus (WSSV) showed a different transcriptome. DCV infection up-regulated the expression levels of 275 genes and down-regulated that of 442 genes significantly and some affected genes were related to phagocytosis. DCV infection activated the JAK/STAT pathway by 1 hour post incubation. The Imd pathway was activated and transcriptional induction of antimicrobial peptides (AMPs) from this pathway was enhanced by 1 hour post incubation. But the Toll pathway was not activated like Imd pathway and the expression levels of AMPs from this pathway was reduced. In addition, most pattern-recognition receptors were inhibited and the antiviral RNAi pathway was not activated in the early stage of DCV infection.
conclusionsIn conclusion, the present study demonstrates that DCV infection may activate phagocytosis, JAK/STAT pathway and Imd pathway in the early host-virus interactions. These results indicate that DCV is capable of activating or inhibiting some immune responses in the host cells and these changes would be vital for virus entry and replication.
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