ArticleAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons2013
Multicenter evaluation of a standardized protocol for noninvasive gene expression profiling.
Article in American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04670926 (Immunosuppression Management in Renal Transplant Recipients With Transplant Excellence Based on TruGraf Test), which is not on this map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Immunosuppression Management in Renal Transplant Recipients With Transplant Excellence Based on TruGraf Test
Who cites it
21 citing papers in PubMed.
- Neuro-Urology and Biobanking: An Integrated Approach for Advancing Research and Improving Patient Care.International journal of molecular sciences · 2023Review
- Successful Islet Transplantation Into a Subcutaneous Polycaprolactone Scaffold in Mice and Pigs.Transplantation direct · 2023Article
- Biomarkers of alloimmune events in pediatric kidney transplantation.Frontiers in pediatrics · 2022Review
- An age-independent gene signature for monitoring acute rejection in kidney transplantation.Theranostics · 2020Article
- Multi-gene technical assessment of qPCR and NanoString n-Counter analysis platforms in cynomolgus monkey cardiac allograft recipients.Cellular immunology · 2020Article
- A Novel Multi-Biomarker Assay for Non-Invasive Quantitative Monitoring of Kidney Injury.Journal of clinical medicine · 2019Article
- Assessment of Postdonation Outcomes in US Living Kidney Donors Using Publicly Available Data Sets.JAMA network open · 2019Article
- A urinary Common Rejection Module (uCRM) score for non-invasive kidney transplant monitoring.PloS one · 2019Article
- Article
- Transplant genetics and genomics.Nature reviews. Genetics · 2017Review
- Moving Biomarkers toward Clinical Implementation in Kidney Transplantation.Journal of the American Society of Nephrology : JASN · 2017Review
- The Use of Genomics and Pathway Analysis in Our Understanding and Prediction of Clinical Renal Transplant Injury.Transplantation · 2016Review
- Quantification of CD4(+) T Cell Alloreactivity and Its Control by Regulatory T Cells Using Time-Lapse Microscopy and Immune Synapse Detection.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2016Article
- Multicenter Analysis of Immune Biomarkers and Heart Transplant Outcomes: Results of the Clinical Trials in Organ Transplantation-05 Study.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2016Observational
- Transplant biopsy beyond light microscopy.BMC nephrology · 2015Review
- Genomic and proteomic fingerprints of acute rejection in peripheral blood and urine.Transplantation reviews (Orlando, Fla.) · 2015Review
- Molecular monitoring of alloimmune-mediated injury in kidney transplant patients.Current opinion in nephrology and hypertension · 2014Review
- Allograft rejection and tubulointerstitial fibrosis in human kidney allografts: interrogation by urinary cell mRNA profiling.Transplantation reviews (Orlando, Fla.) · 2014Review
- Biomarkers for kidney transplant rejection.Nature reviews. Nephrology · 2014Review
- Urinary cell mRNA profiles predictive of human kidney allograft status.Immunological reviews · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
Abstract
Gene expression profiling of transplant recipient blood and urine can potentially be used to monitor graft function, but the multitude of protocols in use make sharing data and comparing results from different laboratories difficult. The goal of this study was to evaluate the performance of current methods of RNA isolation, reverse transcription and quantitative polymerase chain reaction (qPCR) and to test whether multiple centers using a standardized protocol can obtain the same results. Samples, reagents and detailed instructions were distributed to six participating sites that performed RNA isolation, reverse transcription and qPCR for 18S, PRF, GZB, IL8, CXCL9 and CXCL10 as instructed. All data were analyzed at a single site. All sites demonstrated proficiency in RNA isolation and qPCR analysis. Gene expression measurements for all targets and samples had correlations >0.938. The coefficient of variation of fold-changes between pairs of samples was less than 40%. All sites were able to accurately quantify a control sample of known concentration within a factor of 1.5. Collectively, we have formulated and validated detailed methods for measuring gene expression in blood and urine that can yield consistent results in multiple laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.