ArticleMicrobial cell factories2013
Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- The effect of long-term exposure to diatrizoate on microbial communities in activated sludge.Scientific reports · 2025Article
- A Rapid Antibody Enhancement Platform inACS synthetic biology · 2023Article
- The Lipomyces starkeyi gene Ls120451 encodes a cellobiose transporter that enables cellobiose fermentation in Saccharomyces cerevisiae.FEMS yeast research · 2020Article
- Metabolic engineering of the cellulolytic thermophilic fungusBiotechnology for biofuels · 2020Article
- In vivo continuous evolution of genes and pathways in yeast.Nature communications · 2016Article
- Gene Amplification on Demand Accelerates Cellobiose Utilization in Engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2016Article
- Replacement of the initial steps of ethanol metabolism in Saccharomyces cerevisiae by ATP-independent acetylating acetaldehyde dehydrogenase.FEMS yeast research · 2016Article
- Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae.Biotechnology for biofuels · 2016Article
- Enhanced Bioconversion of Cellobiose by Industrial Saccharomyces cerevisiae Used for Cellulose Utilization.Frontiers in microbiology · 2016Article
- Identification of residues important for substrate uptake in a glucose transporter from the filamentous fungus Trichoderma reesei.Scientific reports · 2015Article
- Fungal traits that drive ecosystem dynamics on land.Microbiology and molecular biology reviews : MMBR · 2015Review
- Recent advances in DNA assembly technologies.FEMS yeast research · 2015Review
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories.Computational and structural biotechnology journal · 2014Review
- Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae.Biotechnology for biofuels · 2014Article
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4 authors.
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Abstract
backgroundThe optimization of metabolic pathways is critical for efficient and economical production of biofuels and specialty chemicals. One such significant pathway is the cellobiose utilization pathway, identified as a promising route in biomass utilization. Here we describe the optimization of cellobiose consumption and ethanol productivity by simultaneously engineering both proteins of the pathway, the β-glucosidase (gh1-1) and the cellodextrin transporter (cdt-1), in an example of pathway engineering through directed evolution.
resultsThe improved pathway was assessed based on the strain specific growth rate on cellobiose, with the final mutant exhibiting a 47% increase over the wild-type pathway. Metabolite analysis of the engineered pathway identified a 49% increase in cellobiose consumption (1.78 to 2.65 g cellobiose/(L · h)) and a 64% increase in ethanol productivity (0.611 to 1.00 g ethanol/(L · h)).
conclusionsBy simultaneously engineering multiple proteins in the pathway, cellobiose utilization in S. cerevisiae was improved. This optimization can be generally applied to other metabolic pathways, provided a selection/screening method is available for the desired phenotype. The improved in vivo cellobiose utilization demonstrated here could help to decrease the in vitro enzyme load in biomass pretreatment, ultimately contributing to a reduction in the high cost of biofuel production.
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