Evidence map›Paper›PMID 23801333›Full record

Trial reportThe Journal of biological chemistry2013

Sulfation of the bikunin chondroitin sulfate chain determines heavy chain·hyaluronan complex formation.

Megan S Lord, Anthony J Day, Peter Youssef, Lisheng Zhuo, Hideto Watanabe, Bruce Caterson, John M Whitelock

Open access · hybridAbstract readClinical TrialMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 40 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Expanding the Chondroitin Sulfate Glycoproteome - But How Far?Frontiers in cell and developmental biology · 2021
    Review
  12. Review
  13. The Inter-α-Trypsin Inhibitor Family: Versatile Molecules in Biology and Pathology.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020
    Review
  14. Article
  15. Galactosaminoglycans: Medical Applications and Drawbacks.Molecules (Basel, Switzerland) · 2019
    Review
  16. The journey of hyaluronan research in theThe Journal of biological chemistry · 2019
    Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Megan S LordGraduate School of Biomedical Engineering, The University of New South Wales, Sydney, New South Wales 2052, Australia. m.lord@unsw.edu.au
Anthony J Day
Peter Youssef
Lisheng Zhuo
Hideto Watanabe
Bruce Caterson
John M Whitelock
Aichi Medical University · JPUNSW Sydney · AUCardiff University · GBRoyal Prince Alfred Hospital · AUUniversity of Manchester · GB

Funding

Arthritis Research UK 18472Arthritis Research UK 19489Biotechnology and Biological Sciences Research Council BBS/B/15414Medical Research Council G0701180Versus Arthritis 19858
6 · The paper itself

Abstract

Inter-α-trypsin inhibitor (IαI) is a complex comprising two heavy chains (HCs) that are covalently bound by an ester bond to chondroitin sulfate (CS), which itself is attached to Ser-10 of bikunin. IαI is essential for the trans-esterification of HCs onto hyaluronan (HA). This process is important for the stabilization of HA-rich matrices during ovulation and some inflammatory processes. Bikunin has been isolated previously by anion exchange chromatography with a salt gradient up to 0.5 M NaCl and found to contain unsulfated and 4-sulfated CS disaccharides. In this study, bikunin-containing fractions in plasma and urine were separated by anion exchange chromatography with a salt gradient of 0.1-1.0 M NaCl, and fractions were analyzed for their reactivity with the 4-sulfated CS linkage region antibody (2B6). The fractions that reacted with the 2B6 antibody (0.5-0.8 M NaCl) were found to predominantly contain sulfated CS disaccharides, including disulfated disaccharides, whereas the fractions that did not react with this antibody (0.1-0.5 M NaCl) contained unsulfated and 4-sulfated CS disaccharides. IαI in the 0.5-0.8 M NaCl plasma fraction was able to promote the trans-esterification of HCs to HA in the presence of TSG-6, whereas the 0.1-0.5 M NaCl fraction had a much reduced ability to transfer HC proteins to HA, suggesting that the CS containing 4-sulfated linkage region structures and disulfated disaccharides are involved in the HC transfer. Furthermore, these data highlight that the structure of the CS attached to bikunin is important for the transfer of HC onto HA and emphasize a specific role of CS chain sulfation.

Indexed as

Alpha-GlobulinsChondroitin SulfatesHyaluronic AcidCarbohydrate ConformationExtracellular MatrixFemaleHumansMaleOvulationalpha-1-microglobulinAlpha-GlobulinsChondroitin SulfatesHyaluronic AcidBikuninChondroitin SulfateExtracellular Matrix ProteinsGlycosaminoglycanHyaluronanInter-α-trypsin InhibitorProteoglycanProteoglycan Structure

Identifiers

PMID23801333
PMCPMC3743471
OpenAlexW2086939648

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.