ArticleWorld journal of microbiology & biotechnology2013
Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil.
Article in World journal of microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 65 citations in OpenAlex.
- Exploring new approaches to enhance polyhydroxybutyrate accumulation in Paracoccus denitrificans.World journal of microbiology & biotechnology · 2026Article
- Elucidating carbohydrate preference and engineering glucose transport in Caldimonas thermodepolymerans for enhanced polyhydroxyalkanoate production.Applied microbiology and biotechnology · 2026Article
- Metabolic Engineering Strategies for Enhanced Polyhydroxyalkanoate (PHA) Production inPolymers · 2025Review
- An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer.Microbial biotechnology · 2025Article
- Cultivation driven transcriptomic changes in the wild-type and mutant strains ofComputational and structural biotechnology journal · 2024Article
- Article
- Identification of Oil-LovingPolymers · 2024Article
- Polyhydroxyalkanoate involvement in stress-survival of two psychrophilic bacterial strains from the High Arctic.Applied microbiology and biotechnology · 2024Article
- Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow.Extremophiles : life under extreme conditions · 2023Article
- A Review on EnhancingFrontiers in bioengineering and biotechnology · 2022Review
- Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks.Life (Basel, Switzerland) · 2021Review
- What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?Polymers · 2021Review
- Review
- Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-Materials (Basel, Switzerland) · 2020Article
- Fed-Batch Synthesis of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) from Sucrose and 4-Hydroxybutyrate Precursors by Burkholderia sacchari Strain DSM 17165.Bioengineering (Basel, Switzerland) · 2017Article
- US132 Cyclodextrin Glucanotransferase Engineering by Random Mutagenesis for an Anti-Staling Purpose.Molecular biotechnology · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADP⁺ ratio. It is likely that as a side effect of the increase of NADPH/NADP⁺ ratio the activity of PHB biosynthetic pathway was enhanced, which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporate propionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and the mutant, respectively)). We assume that it may be caused by lower availability of oxaloacetate for the utilization of propionyl-CoA in 2-methylcitrate cycle due to increased action of malic enzyme. Therefore, propionyl-CoA was incorporated into copolymer rather than transformed to pyruvate via 2-methylcitrate cycle. Thus, the mutant was capable of the utilization of waste frying oils and the production of P(3HB-co-3HV) with better yields and improved content of 3HV resulting in better mechanical properties of copolymer than the wild-type strain. The results of this work may be used for the development of innovative fermentation strategies for the production of PHA and also it might help to define novel targets for the genetic manipulations of PHA producing bacteria.
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