ArticleGenome biology2013
Mapping gene activity of Arabidopsis root hairs.
Article in Genome biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Root hair growth from the pH point of view.Frontiers in plant science · 2022Pooled it
- Single-cell transcriptomic analysis of plant quiescent center by third-generation sequencing reveals developmental trajectories.Genome biology · 2026Article
- Linking micro-X-ray fluorescence spectroscopy and X-ray computed tomography with model simulation explains differences in nutrient gradients around roots of different types and ages.The New phytologist · 2025Article
- Article
- Protein Phosphorylation Orchestrates Acclimations of Arabidopsis Plants to Environmental pH.Molecular & cellular proteomics : MCP · 2024Article
- New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics.Plants (Basel, Switzerland) · 2023Article
- Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq.STAR protocols · 2023Article
- Uncovering gene-family founder events during major evolutionary transitions in animals, plants and fungi using GenEra.Genome biology · 2023Article
- Article
- Multilayered regulation of iron homeostasis in Arabidopsis.Frontiers in plant science · 2023Review
- Two zinc finger proteins with functions in mNature communications · 2022Article
- Root hair-specific transcriptome reveals response to low phosphorus inFrontiers in plant science · 2022Article
- Identification and analysis of proline-rich proteins and hybrid proline-rich proteins super family genes fromFrontiers in plant science · 2022Article
- Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner inInternational journal of molecular sciences · 2020Article
- Estimating the importance of maize root hairs in low phosphorus conditions and under drought.Annals of botany · 2019Article
- Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Roles of plasma membrane proton ATPases AHA2 and AHA7 in normal growth of roots and root hairs in Arabidopsis thaliana.Physiologia plantarum · 2019Article
- Contribution of Root Hair Development to Sulfate Uptake inPlants (Basel, Switzerland) · 2019Article
- Identification and mapping of two independent recessive loci for the root hairless mutant phenotype in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Root Hair Single Cell Type Specific Profiles of Gene Expression and Alternative Polyadenylation Under Cadmium Stress.Frontiers in plant science · 2019Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative information on gene activity at single cell-type resolution is essential for the understanding of how cells work and interact. Root hairs, or trichoblasts, tubular-shaped outgrowths of specialized cells in the epidermis, represent an ideal model for cell fate acquisition and differentiation in plants.
resultsHere, we provide an atlas of gene and protein expression in Arabidopsis root hair cells, generated by paired-end RNA sequencing and LC/MS-MS analysis of protoplasts from plants containing a pEXP7-GFP reporter construct. In total, transcripts of 23,034 genes were detected in root hairs. High-resolution proteome analysis led to the reliable identification of 2,447 proteins, 129 of which were differentially expressed between root hairs and non-root hair tissue. Dissection of pre-mRNA splicing patterns showed that all types of alternative splicing were cell type-dependent, and less complex in EXP7-expressing cells when compared to non-root hair cells. Intron retention was repressed in several transcripts functionally related to root hair morphogenesis, indicative of a cell type-specific control of gene expression by alternative splicing of pre-mRNA. Concordance between mRNA and protein expression was generally high, but in many cases mRNA expression was not predictive for protein abundance.
conclusionsThe integrated analysis shows that gene activity in root hairs is dictated by orchestrated, multilayered regulatory mechanisms that allow for a cell type-specific composition of functional components.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.