ArticleTissue engineering. Part C, Methods2014
Three-dimensional characterization of tissue-engineered constructs by contrast-enhanced nanofocus computed tomography.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- RETRACTED: ThePolymers · 2023Article
- Monitoring the Remodeling of Biohybrid Tissue-Engineered Vascular Grafts by Multimodal Molecular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Application of medical imaging methods and artificial intelligence in tissue engineering and organ-on-a-chip.Frontiers in bioengineering and biotechnology · 2022Review
- Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications.Materials (Basel, Switzerland) · 2021Review
- Quality control methods in musculoskeletal tissue engineering: from imaging to biosensors.Bone research · 2021Review
- A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System.International journal of molecular sciences · 2021Review
- Fluid flow-induced cell stimulation in bone tissue engineering changes due to interstitial tissue formation in vitro.International journal for numerical methods in biomedical engineering · 2020Article
- Computational Modeling of Human Mesenchymal Stromal Cell Proliferation and Extra-Cellular Matrix Production in 3D Porous Scaffolds in a Perfusion Bioreactor: The Effect of Growth Factors.Frontiers in bioengineering and biotechnology · 2020Article
- Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Perspective.International journal of bioprinting · 2019Article
- New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.Biotechnology letters · 2019Review
- Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy.Biomaterials · 2017Article
- Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid.Contrast media & molecular imaging · 2017Article
- Quantitative Validation of the Presto Blue Metabolic Assay for Online Monitoring of Cell Proliferation in a 3D Perfusion Bioreactor System.Tissue engineering. Part C, Methods · 2015Article
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
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6 authors.
Funding
Abstract
To successfully implement tissue-engineered (TE) constructs as part of a clinical therapy, it is necessary to develop quality control tools that will ensure accurate and consistent TE construct release specifications. Hence, advanced methods to monitor TE construct properties need to be further developed. In this study, we showed proof of concept for contrast-enhanced nanofocus computed tomography (CE-nano-CT) as a whole-construct imaging technique with a noninvasive potential that enables three-dimensional (3D) visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular, we performed a 3D qualitative and quantitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely, Hexabrix® and phosphotungstic acid (PTA). To evaluate the potential of CE-nano-CT, a comparison was made to standardly used techniques such as Live/Dead viability/cytotoxicity, Picrosirius Red staining, and to net dry weight measurements of the TE constructs. When using Hexabrix as the contrast agent, the ECM volume fitted linearly with the net dry ECM weight independent from the flow rate used, thus suggesting that it stains most of the ECM. When using PTA as the contrast agent, comparing to net weight measurements showed that PTA only stains a part of the ECM. This was attributed to the binding specificity of this contrast agent. In addition, the PTA-stained CE-nano-CT data showed pronounced distinction between flow conditions when compared to Hexabrix, indicating culture-specific structural ECM differences. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of TE constructs in well-characterized TE products.
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