ArticleBMC genomics2013
Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis).
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Lawsonia intracellularis regulates nuclear factor-κB signalling pathway during infection.PloS one · 2024Article
- Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis.Veterinary research · 2023Article
- Microbial Pathogenesis in the Era of Spatial Omics.Infection and immunity · 2023Review
- Alleviative Effect of Probiotic Ferment onBiology · 2023Article
- Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis.Veterinary research communications · 2022Article
- Laser capture microdissection to study Bacillus cereus iron homeostasis gene expression during Galleria mellonella in vivo gut colonization.Virulence · 2021Article
- Review of methods for the detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Review
- Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity.Veterinary research · 2021Article
- Best practices on the differential expression analysis of multi-species RNA-seq.Genome biology · 2021Review
- Preparation and Characterization of a New Monoclonal Antibody Specific AgainstFrontiers in veterinary science · 2021Article
- Spatially distinct physiology of Bacteroides fragilis within the proximal colon of gnotobiotic mice.Nature microbiology · 2020Article
- Metagenomic sequencing of clinical samples reveals a single widespread clone ofMicrobial genomics · 2020Article
- Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq.Scientific reports · 2019Article
- Nutrient digestibility, organ morphometry and performance in vaccinated or non-vaccinated Lawsonia intracellularis infected piglets.BMC veterinary research · 2018Article
- Article
- Review
- Aligner optimization increases accuracy and decreases compute times in multi-species sequence data.Microbial genomics · 2017Article
- Resolving host-pathogen interactions by dual RNA-seq.PLoS pathogens · 2017Review
- A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host CellsFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLawsonia intracellularis is an obligate intracellular bacterium and the etiologic agent of proliferative enteropathy. The disease is endemic in pigs, emerging in horses and has been described in various other species including nonhuman primates. Cell proliferation is associated with bacterial replication in enterocyte cytoplasm, but the molecular basis of the host-pathogen interaction is unknown. We used laser capture microdissection coupled with RNA-seq technology to characterize the transcriptional responses of infected enterocytes and the host-pathogen interaction.
resultsProliferative enterocytes was associated with activation of transcription, protein biosynthesis and genes acting on the G1 phase of the host cell cycle (Rho family). The lack of differentiation in infected enterocytes was demonstrated by the repression of membrane transporters related to nutrient acquisition. The activation of the copper uptake transporter by infected enterocytes was associated with high expression of the Zn/Cu superoxide dismutase by L. intracellularis. This suggests that the intracellular bacteria incorporate intracytoplasmic copper and express a sophisticated mechanism to cope with oxidative stress.
conclusionsThe feasibility of coupling microdissection and RNA-seq was demonstrated by characterizing the host-bacterial interactions from a specific cell type in a heterogeneous tissue. High expression of L. intracellularis genes encoding hypothetical proteins and activation of host Rho genes infers the role of unrecognized bacterial cyclomodulins in the pathogenesis of proliferative enteropathy.
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