ArticleTissue engineering. Part A2013
Enhanced osteogenesis in cocultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues.Materials today. Bio · 2024Article
- Research progress on the regulatory mechanism of integrin-mediated mechanical stress in cells involved in bone metabolism.Journal of cellular and molecular medicine · 2024Review
- A Comprehensive Look at In Vitro Angiogenesis Image Analysis Software.International journal of molecular sciences · 2023Review
- Recent Advances in Enhancement Strategies for Osteogenic Differentiation of Mesenchymal Stem Cells in Bone Tissue Engineering.Frontiers in cell and developmental biology · 2022Review
- Bone Microvasculature: Stimulus for Tissue Function and Regeneration.Tissue engineering. Part B, Reviews · 2021Review
- Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis.Materials (Basel, Switzerland) · 2020Article
- Titania Nanofiber Scaffolds with Enhanced Biointegration Activity-Preliminary In Vitro Studies.International journal of molecular sciences · 2019Article
- 3D Human Periodontal Stem Cells and Endothelial Cells Promote Bone Development in Bovine Pericardium-Based Tissue Biomaterial.Materials (Basel, Switzerland) · 2019Article
- Bioprinted osteon-like scaffolds enhance in vivo neovascularization.Biofabrication · 2019Article
- Analysis of the Intrinsic Self-Organising Properties of Mesenchymal Stromal Cells in Three-Dimensional Co-Culture Models with Endothelial Cells.Bioengineering (Basel, Switzerland) · 2018Article
- Angiogenic and Osteogenic Synergy of Human Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured on a Nanomatrix.Scientific reports · 2018Article
- Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model.Scientific reports · 2018Article
- Co-Culture with Human Osteoblasts and Exposure to Extremely Low Frequency Pulsed Electromagnetic Fields Improve Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2018Article
- Honing Cell and Tissue Culture Conditions for Bone and Cartilage Tissue Engineering.Cold Spring Harbor perspectives in medicine · 2017Review
- Strategies to retain properties of bone marrow-derived mesenchymal stem cells ex vivo.Annals of the New York Academy of Sciences · 2017Review
- Biomaterial-mediated strategies targeting vascularization for bone repair.Drug delivery and translational research · 2016Review
- Review
- Fabrication of Biomimetic Bone Tissue Using Mesenchymal Stem Cell-Derived Three-Dimensional Constructs Incorporating Endothelial Cells.PloS one · 2015Article
- Article
- 3D tissue-engineered model of Ewing's sarcoma.Advanced drug delivery reviews · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In this work, human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly cocultured with human umbilical vein endothelial cells (HUVECs) on electrospun three-dimensional poly(ɛ-caprolactone) microfiber scaffolds to evaluate the coculture's effect on the generation of osteogenic constructs. Specifically, cells were cultured on scaffolds for up to 3 weeks, and the cellularity, alkaline phosphatase (ALP) activity, and bone-like matrix formation were assessed. Constructs with cocultures and monocultures had almost identical cellularity after the first week, however, lower cellularity was observed in cocultures compared to monocultures during the subsequent 2 weeks of culture. Scaffolds with cocultures showed a significantly higher ALP activity, glycosaminoglycan and collagen production, as well as greater calcium deposition over the course of study compared to monocultures of hMSCs. Furthermore, the osteogenic outcome was equally robust in cocultures containing osteogenically precultured and non-precultured hMSCs. The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic culture conditions is an effective strategy to promote osteogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.