ArticlePloS one2013
Evaluation of small intestine grafts decellularization methods for corneal tissue engineering.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 112 citations in OpenAlex.
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- Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Characterization of Decellularized Extracellular Matrix from Milkfish (Biomimetics (Basel, Switzerland) · 2022Article
- Development of decellularization protocol for caprine small intestine submucosa as a biomaterial.Biomaterials and biosystems · 2022Article
- Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods.Frontiers in bioengineering and biotechnology · 2022Review
- Small intestinal submucosa-derived extracellular matrix as a heterotopic scaffold for cardiovascular applications.Frontiers in bioengineering and biotechnology · 2022Article
- Preconditioning process for dermal tissue decellularization using electroporation with sonication.Regenerative biomaterials · 2022Article
- Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering.Biomedicines · 2021Article
- A review of biomimetic scaffolds for bone regeneration: Toward a cell-free strategy.Bioengineering & translational medicine · 2021Review
- Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering.Journal of nanobiotechnology · 2020Article
- Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy.Scientific reports · 2020Article
- Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.International journal of molecular sciences · 2020Review
- In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications. Running Title: Fibrin-Agarose Functionalized Scaffolds.Materials (Basel, Switzerland) · 2020Article
- Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in the development of cornea substitutes by tissue engineering techniques have focused on the use of decellularized tissue scaffolds. In this work, we evaluated different chemical and physical decellularization methods on small intestine tissues to determine the most appropriate decellularization protocols for corneal applications. Our results revealed that the most efficient decellularization agents were the SDS and triton X-100 detergents, which were able to efficiently remove most cell nuclei and residual DNA. Histological and histochemical analyses revealed that collagen fibers were preserved upon decellularization with triton X-100, NaCl and sonication, whereas reticular fibers were properly preserved by decellularization with UV exposure. Extracellular matrix glycoproteins were preserved after decellularization with SDS, triton X-100 and sonication, whereas proteoglycans were not affected by any of the decellularization protocols. Tissue transparency was significantly higher than control non-decellularized tissues for all protocols, although the best light transmittance results were found in tissues decellularized with SDS and triton X-100. In conclusion, our results suggest that decellularized intestinal grafts could be used as biological scaffolds for cornea tissue engineering. Decellularization with triton X-100 was able to efficiently remove all cells from the tissues while preserving tissue structure and most fibrillar and non-fibrillar extracellular matrix components, suggesting that this specific decellularization agent could be safely used for efficient decellularization of SI tissues for cornea TE applications.
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