ArticlePloS one2013
rAAV-mediated subcellular targeting of optogenetic tools in retinal ganglion cells in vivo.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed.
- Voltage imaging: A practical guide.Neuron · 2026Review
- Optogenetic Vision Restoration.Cold Spring Harbor perspectives in medicine · 2024Review
- Whole-eye transplantation: Current challenges and future perspectives.World journal of transplantation · 2024Review
- Contribution of optical resolution to the spatial precision of two-photon optogenetic photostimulationNeurophotonics · 2024Article
- Nanoparticle-based optical interfaces for retinal neuromodulation: a review.Frontiers in cellular neuroscience · 2024Review
- Starburst amacrine cells amplify optogenetic visual restoration through gap junctions.Molecular therapy. Methods & clinical development · 2023Article
- Cellular and subcellular optogenetic approaches towards neuroprotection and vision restoration.Progress in retinal and eye research · 2023Review
- All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse.Cell reports · 2023Article
- Transient photocurrents in a subthreshold evidence accumulator accelerate perceptual decisions.Nature communications · 2023Article
- Application of optogenetic glial cells to neuron-glial communication.Frontiers in cellular neuroscience · 2023Review
- Optogenetic Therapy for Visual Restoration.International journal of molecular sciences · 2022Review
- Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022Article
- Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Emerging strategies for the genetic dissection of gene functions, cell types, and neural circuits in the mammalian brain.Molecular psychiatry · 2022Review
- Probing neural codes with two-photon holographic optogenetics.Nature neuroscience · 2021Review
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- An engineered channelrhodopsin optimized for axon terminal activation and circuit mapping.Communications biology · 2021Article
- Autonomous Purkinje cell activation instructs bidirectional motor learning through evoked dendritic calcium signaling.Nature communications · 2021Article
- Sensitization of ON-bipolar cells with ambient light activatable multi-characteristic opsin rescues vision in mice.Gene therapy · 2021Article
- Optogenetic Strategies for Vision Restoration.Advances in experimental medicine and biology · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Expression of optogenetic tools in surviving inner retinal neurons to impart retinal light sensitivity has been a new strategy for restoring vision after photoreceptor degeneration. One potential approach for restoring retinal light sensitivity after photoreceptor degeneration is to express optogenetic tools in retinal ganglion cells (RGCs). For this approach, restoration of ON and OFF center-surround receptive fields in RGCs, a key feature of visual information processing, may be important. A possible solution is to differentially express depolarizing and hyperpolarizing optogenetic tools, such as channelrhodopsin-2 and halorhodopsin, to the center and peripheral regions of the RGC dendritic field by using protein targeting motifs. Recombinant adeno-associated virus (rAAV) vectors have proven to be a powerful vehicle for in vitro and in vivo gene delivery, including in the retina. Therefore, the search for protein targeting motifs that can achieve rAAV-mediated subcellular targeted expression would be particularly valuable for developing therapeutic applications. In this study, we identified two protein motifs that are suitable for rAAV-mediated subcellular targeting for generating center-surround receptive fields while reducing the axonal expression in RGCs. Resulting morphological dendritic field and physiological response field by center-targeting were significantly smaller than those produced by surround-targeting. rAAV motif-mediated protein targeting could also be a valuable tool for studying physiological function and clinical applications in other areas of the central nervous system.
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Registered trials
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